How do you change the pH of a HEPES buffer?
How do you change the pH of a HEPES buffer? Prepare 800 mL of dH2O in a suitable container. Add 238.3 g of HEPES to the solution. Adjust solution to desired pH by 10N NaOH. Add dH2O until volume is 1 L. What pH should HEPES be? 6.8 to 8.2 HEPES has been described as […]