How does DMSO affect annealing temperature?

How does DMSO affect annealing temperature? If high DMSO concentration is used, the annealing temperature must be lowered, as DMSO decreases the melting point of the primers. It has been reported that 10% DMSO decreases the annealing temperature by 5.5-6.0°C. What temperature should annealing PCR be? between 48-72°C The annealing temperature (typically between 48-72°C) is […]

What is bad about capital punishment?

What is bad about capital punishment? It is the ultimate cruel, inhuman and degrading punishment. The death penalty is discriminatory. It is often used against the most vulnerable in society, including the poor, ethnic and religious minorities, and people with mental disabilities. Some governments use it to silence their opponents. Is capital punishment an ethical […]

What causes deep vein thrombosis in pregnancy?

What causes deep vein thrombosis in pregnancy? During pregnancy, factors that increase the risk of developing DVT include varicose veins, diabetes multiple gestations, and more. The most common type of venous thromboembolism (VTE) is deep vein thrombosis (DVT), which refers to blood clots in the deep veins of the leg, arm, or pelvis. How can […]

How does home detention work in South Australia?

How does home detention work in South Australia? Home detention allows offenders and prisoners to live in the community under strict supervision at a nominated location. The offender must not leave that address without approval. The location of the person on Home Detention is strictly monitored by an electronic monitoring device. How do you qualify […]

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