Is DsRed a Monomer?
Is DsRed a Monomer?
DsRed-Monomer is an ideal fusion tag because it is a true monomer and for this reason has been expressed as a fusion with a large panel of diverse proteins with diverse functions and subcellular locations.
What is DsRed used for?
DsRed is an interesting marker protein for use in plants because of its red-shifted wavelength spectrum that will avoid damaging cells and tissues by excitation light.
What wavelength is DsRed?
583 nm
DrFP583 (DsRed) is a bright red fluorescent protein cloned from reef coral (Matz et al. 1999) and has excitation and emission maxima at 558 and 583 nm, respectively.
What is the number of amino acids and the molecular weight of the in the DsRed protein?
DsRed, a 28-kDa polypeptide, has essentially the same chromophore as avGFP, autocatalytically formed from an internal Gln-Tyr-Gly (residues 66–68) tripeptide (amino acid sequence numbering of wild-type protein) (4)….Abbreviations.
| FPs | fluorescent proteins |
|---|---|
| avGFPs | Aequorea victoria green FPs |
What is the molecular weight of DsRed?
26.8 kDa
The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa.
What is a tandem dimer?
Brightness and. Stabilit y. tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifically designed for low aggregation (1).
Is DsRed a RFP?
Since the discovery of the first red fluorescent protein (RFP), named DsRed, 12 years ago, a wide pallet of red-shifted fluorescent proteins has been cloned and biotechnologically developed into monomeric fluorescent probes for optical microscopy.
Where is DsRed from?
Discosoma sea anemones
DsRed is isolated from Discosoma sea anemones, and is a tetrameric protein. Most red fluorescent proteins come from DsRed. DsRed has low photostability (resistance to change under the influence of radiant energy or light) and a slow maturation rate (time until half the protein is folded).
Is DsRed the same as RFP?
Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red.
Is the DsRed-monomer patented in the US?
The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511 and 7,393,923. pDsRed-Monomer-Actin encodes DsRed-Monomer, a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed.
What is rdsred-monomer used for?
Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed-Monomer) can be used as a standard for protein gels, Western blots, and fluorometry. It can also be used as a control for expression studies and for microinjection into cells and tissues.
What is the DsRed-monomer sequence?
The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. The DsRed-Monomer fluorescent protein is extremely stable, allowing you to monitor fluorescence over extended periods of time.
Is DsRed monomer a fusion tag?
DsRed-Monomer fluorescent protein. DsRed-Monomer is an ideal fusion tag because it is a true monomer and for this reason has been expressed as a fusion with a large panel of diverse proteins with diverse functions and subcellular locations.
