What does Enterotube II test for?
What does Enterotube II test for?
The Enterotube II is a compartmental plastic tube containing 12 media to test for: acid and gas production from glucose, lysine decarboxylase, ornithine decarbox- ylase, H2S, indole, acetoin (VP test), phenylalanine de- aminase, urease, citrate utilization, and acid production from adonitol, lactose, arabinose.
What type of bacteria does Enterotube identify?
The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae.
How is Enterotube used to identify microorganisms?
The enclosed inoculating wire allows inoculation of all compartments in one step from one or a few single colonies of your unknown microorganism. The resulting combination of enterotube reactions, together with other metabolism tests can help you to identify unknown organisms.
How do you read the EnteroPluri-test?
Lay EnteroPluri-Test with its flat surface pointing upward and, by punching the plastic film, add 3 or 4 drops of Kovac’s Indole Reagent in the sector H2S/Indole. The reaction is positive if a pink-red colour develops in the added reagent within 10-15 seconds.
What are the advantages of EnteroPluri-test?
Principle of the method: EnteroPluri-Test makes possible the identification of the Enterobacteriaceae and other gram negative, oxidase negative bacteria isolated from clinical and environmental samples. The identification is based on biochemical tests performed on culture media containing specific substrates.
How many compartments does the Enterotube II have?
The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae. It consists of a tube with a flat side and contains 12 compartments for different biochemical tests.
What is the purpose of carbohydrate fermentation test?
The carbohydrate fermentation test is used to determine whether or not a bacteria can utilize a certain carbohydrate. It tests for the presence of acid and/or gas produced from the fermentation of a single particular carbohydrate.
What are the advantages of the EnteroPluri-test?
How do you test for Enterobacter cloacae?
The most important test to document Enterobacter infections is culture. Direct Gram staining of the specimen is also very useful because it allows rapid diagnosis of an infection caused by gram-negative bacilli and helps in the selection of antibiotics with known activity against most of these bacteria.
How is the EnteroPluri test system inoculated?
Inoculate EnteroPluri-Test by first twisting wire, then withdrawing wire through all twelve compartments applying a turning motion Reinsert wire into EnteroPluri-Test, using a turning motion through all twelve compartments, until the notch on the wire is aligned with the opening of the tube.
How do I interpret the results of BBL Enterotube II?
The Results Pad and Color Reaction Chart permit a rapid check of the positive reactions obtained with BBL Enterotube II. The checked positive numbers are totaled, and the composite number is then located in the Interpretation Guide to identify the organisms.
What is Enterotube 2 used for?
The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase – negative, gram – negative, rod shaped bacteria of the family Enterobacteriaceae. It consists of a tube with a flat side and contains 12 compartments for different biochemical tests.
How accurate is the Enterotube II at identifying unknown bacterial cultures?
In one study, the first testing of the Enterotube™ II correctly identified only 90.6% of the unknown bacterial cultures. Repeat testing using the Enterotube™ II identified 97.9% of the bacterial cultures. (a total of 235 organisms were used) .
How long does it take for a PCR tube to incubate?
The tube is finally recapped and incubated at 37 degrees Celsius for 24 hours. After 24 hours of incubation, any color change is recorded in the ID card provided by the manufacturer or from a separate vendor. The appropriate reagents are added to the indole and Voges-Proskauer compartments and their results are recorded in the same ID card.
