What is the color reaction of cannabis with fast blue salt?
What is the color reaction of cannabis with fast blue salt?
The principal screening test used by the Brazilian Forensic Police is a colorimetric test called Fast Blue B or BB salt, in which the development of a reddish color in a basic medium indicates the presence of cannabinoids such as Δ9-THC, cannabinol (CBN) and/or cannabidiol (CBD).
How does Fast Blue b work?
Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye.
What is the chemical name of fast blue salt?
Fast Blue B Salt
| PubChem CID | 84305 |
|---|---|
| Chemical Safety | Laboratory Chemical Safety Summary (LCSS) Datasheet |
| Molecular Formula | C14H12Cl4N4O2Zn |
| Synonyms | Fast Blue B Salt 14263-94-6 Fast Blue Salt B Azoic diazo 48 EINECS 238-153-2 More… |
| Molecular Weight | 475.5 |
What is fast blue BB?
Thermo Scientific™ Fast Blue BB salt Fast Blue BB salt is used as a general substrate for β-glycosidase. Two moles of 6-bromo-2-napthol-β-d-glucopyranoside are cleaved by β-glucosidases into 2 moles of d-glucose and 2 moles of 6-bromo-2-napthol.
How many cannabinoids are identified?
How many cannabinoids are there? Besides THC and CBD, more than 100 other cannabinoids have been identified.
How do you make a Duquenois reagent?
Process. The reagent can be prepared by adding 2 grams of vanillin and 2.5 milliliters of acetaldehyde to 100 milliliters of ethanol. The test is performed by placing approximately 10 to 20 milligrams of a target substance in a glass test tube, then 10 drops of the Duquenois reagent.
What does Luxol fast blue stain?
Luxol fadt blue stain (LFB) is used to visualize myelin in nerve tissue. Thanks to this staining, it is possible to visualize the myelin and the fatty substance of the brain called white matter which includes the axons of the nerve cells.
What is the Duquenois-Levine test?
The Duquenois reagent is used in the Rapid Modified Duquenois–Levine test (also known as the simple Rapid Duquenois Test), is an established screening test for the presence of cannabis.
What is the purpose of the Duquenois-Levine test?
The Duquenois-Levine test for tetrahydrocannabinol (THC) has been used in the forensic science community for over 80 years. This three-part color test is one of many tests used to confirm the presence of THC.
Does myelination increase resistance?
Myelination improves conduction by increasing the membrane resistance and decreasing the membrane capacitance. There are periodic gaps along a myelinate axon where there is no myelin and the axonal membrane is exposed.
What does white matter do in the brain?
White matter is tissue in the brain composed of nerve fibers. The fibers (called axons) connect nerve cells and are covered by myelin (a type of fat). The myelin is what gives white matter its white color. Myelin speeds up the signals between the cells, enabling the brain cells to quickly send and receive messages.
What does the Scott test detect the presence of?
The Scott test is a preliminary colorimetric method to analyze cocaine. A blue color result in the final step denotes a positive indication for cocaine; however, some pharmacological products may lead to false positives when concentration is higher than 1 mg.
