How will you perform assay of streptomycin?
How will you perform assay of streptomycin?
SUMMARY: -4 dilution method for the assay of streptomycin’ is described using a digest nutrient broth as the test medium and a certain strain of Escherichia coEi (Bacterium coli) as the test organism. With dilution of the medium the sensitivity of the test is increased while its accuracy is decreased.
Which microorganism is used for microbiological assay of streptomycin?
For the assay of Streptomycin, U.S.P. advices turbidimetric method (11), using 5-7 ml. ofthe suspension of the test organism Klebsiella pneumoniae, having a light transmission reading 65% in a suitable electrophotometer, fitted with a 650 m(J. filter, in 100ml.
What are the methods of microbiological assay?
There are three main methods of microbiological assay by which the potency of samples and standard solution may be compared: (1) dilution methods, (2) turbidimetric, titrimetric, and gravimetric methods, and (3) diffusion methods. Microbiological assays are called dilution assays.
What is microbiological assay?
Microbiological Assay. Introduction • A microbiological assay defined as qualitative or quantitative determination of chemical compound from a simple or even complex material with the use of microorganisms.
Which organism is used for microbial assay of chloramphenicol?
An agar diffusion assay for chloramphenicol and tetracyclines is described, using inhibition of hemolysis by Clostridium perfringens as a biological indicator of antimicrobial activity. Well-defined zones formed after a 1.5- to 2-h incubation in an anaerobic chamber or after 3 h using a GasPak system.
What is turbidimetric method in microbiology?
The turbidimetric method is characterized by a series of tubes containing different concentrations of antibiotics in a liquid culture medium inoculated with the test microorganism. Reading data obtained after incubation provides a curve displaying the antibiotic concentration with turbidity.
Which assay is the most accurate method for microbial assay of antibiotics?
The agar diffusion method (Cylindrical-plate or Cup-plate) is a most widely employed method for the estimation of potency and bioactivity of antibiotics. The agar diffusion method depends on diffusion of the antibiotic from a vertical cylinder through a solidified agar layer in a Petri dish.
Why microbiological assay is important?
Microbiological assays are prescribed for the drug substances and preparations where the potency cannot be adequately determined by chemical means. Additionally, the resistance and sensitivity of pathogenic microorganisms is also determined by microbiological assays.
What is analytical techniques in microbiology?
Analytical microbiology may be defined as that. branch of microbiology in which microorganisms are. used as reagents for the quantitative determination of. certain chemical compounds.
What is the principle of microbiological assay?
The microbiological assay is based upon a comparison of the inhibition of growth of micro-organisms by measured concentration of the antibiotics to be examined with that produced by known concentrations of a standard preparation of the antibiotic having a known activity.
Which organism is used for assay of tetracycline?
Staphylococcus aureus ATCC 9144 and NCTC 7447, B. subtilis ATCC 6633, B. cereus ATCC 11778 and E. hirae ATCC 10541 are used as test organisms in different microbiological methods for quantitative assay of tetracyclines [2, 7, 8, 11].
Which microorganism is used for the assay of tetracycline antibiotic?
Abstract. An agar diffusion assay for chloramphenicol and tetracyclines is described, using inhibition of hemolysis by Clostridium perfringens as a biological indicator of antimicrobial activity. Well-defined zones formed after a 1.5- to 2-h incubation in an anaerobic chamber or after 3 h using a GasPak system.
How is streptomycin prepared from its salts?
Streptomycin and dihydrostreptomycin are basic compounds and therefore, they are usually prepared as salts. Streptomycin is present as the hydrochloride as crystalline HCl double salt with calcium chorial or as phosphate or sulfate and dihydrostreptomycin like hydrochloride or sulfate. Woodruff and McDaniel (1954).
What is the solubility of streptomycin Sesquisulfate?
White to light gray or pale buff powder; faint amine-like odor; solubility in mg/mL at about 28 °C: water greater than 20; methanol 0.85; ethanol 0.30; isopropanol 0.01; petroleum ether 0.015; carbon tetrachloride 0.035; ether 0.035, Practically insoluble in chloroform. / Streptomycin sesquisulfate /
What is the molecular formula of streptomycin?
Streptomycin PubChem CID 19649 Structure Find Similar Structures Chemical Safety Laboratory Chemical Safety Summary (LCSS Molecular Formula C21H39N7O12 Synonyms streptomycin 57-92-1 Streptomycin A Stre
What is the peak concentration of streptomycin?
Following IM administration of a single 1-g dose of streptomycin in adults with normal renal function, peak serum streptomycin concentrations are attained within 1 hour and range from 25-50 ug/mL; serum concentrations decrease 50% by 5-6 hours after the dose.
