How do you make Janus Green B stain?
How do you make Janus Green B stain?
Solution Prep To prepare a Janus Green B solution, use a 1% aqueous solution. Add 1 g of Janus Green B to 75 ml of DI water. Dilute to a final volume of 100 ml with DI water.
What is the Colour of Janus Green B?
Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells.
Is Janus Green B is a blue dye?
Janus Green B is a basic dye and vital stain used in histology. The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour.
What is the role of Janus Green B stain in mitochondria slide preparation?
Explanation: The Janus Green B is a stain which acts as a indicator. The indicator is used to stain the mitochondria in the living cell. It is also used to stain nucleic acid, chromosomes and biomolecules.
What is the use of safranin solution?
Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red. This is the classic counterstain in both Gram stains and endospore staining. It can also be used for the detection of cartilage, mucin and mast cell granules.
What is used for staining mitochondria?
Answer: (d) Janus green is used to stain mitochondria. Janus green act as an indicator and changes colour according to the amount of oxygen present.
Which stain is used in mitochondria staining technique?
The Janus green is a basic dye which is also used as a vital stain in histology. This dye is used for staining the mitochondria.
What does fast green stain?
fast green A green dye used in optical microscopy that stains cellulose, cytoplasm, collagen, and mucus green. It is frequently used to stain plant tissues, with safranin as a counterstain.
What does methylene blue stain?
What does methylene blue stain? Methylene blue, also known as methylthioninium chloride, can be used to stain or highlight parts of animal, bacteria, and blood tissue specimens in microbiology. Use it to closely observe the shapes, structures, and locations of dead cells and other tissues!
What is the meaning of Janus Green?
Definition of Janus green : a basic azine dye used especially as a vital biological stain (as for mitochondria)
What is the role of Janus green B Indicator in anaerobic experiment with yeast?
Answer: The indicator Janus Green B changes colour according to the amount of oxygen present. In the absence of oxygen, the indicator is reduced and changes to a pink colour.
What is the role of Janus?
Janus kinases (JAKs) consist of a group of intracellular tyrosine kinases that transmit signals from cytokine or growth factor–receptor interactions on the cellular membrane to influence cellular processes of hematopoiesis and immune cell function.
What is Janus Green B stain used for?
Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. The indicator Janus Green B changes colour according to the amount of oxygen present.
How does the indicator Janus Green B change colour?
The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour. ^ Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula.
What is the use of indicator Janus Green B in mitochondria?
These are also used for staining mitochondria where there is change in color of indicator janus green B depending upon the amount of oxygen present. The indicator is oxidized in blue color in presence of oxygen and shows pink color in case of absence of oxygen. Yes! I am Interested
