What is the sample volume if the blood is withdrawn from retro-orbital plexus in recovery rate?
What is the sample volume if the blood is withdrawn from retro-orbital plexus in recovery rate?
The maximum amount of blood that may be withdrawn at one time from this location is 1% of the animal’s body weight (e.g., 0.2 ml from a 20 gm adult mouse and 2.0 ml’s from a 200 gm adult rat). Blood can only be collected once per week from one eye. Subsequent bleeds should use alternate eyes.
What is retro-orbital plexus?
The retro-orbital sinus (mouse) and plexus (rat) is a system of dilated venous channels at the back of the orbit. • Blood can be collected from this area in anesthetized animals using a sterile hematocrit tube.
How do you get a blood sample from a rat?
Blood is collected using a 23 gauge needle from the lateral saphenous vein. Blood flow is stopped by applying pressure with sterile gauze to achieve hemostasis. No more than four blood samples should be taken within any 24-hour period. For multiple samples the scab or the blood clot should be removed.
How do you collect blood from a rat?
What is a retroorbital blood collection?
Retro‐orbital bleeding is a method of blood collection whereby the retro‐orbital sinus in mice or the retro‐orbital plexus in rats is penetrated with a capillary tube. Retro‐orbital sampling has a greater potential than other blood collection routes to result in complications.
How do you use retroorbital sampling in rats?
Retro-orbital sampling can be used in both mice and rats by penetrating the retro-orbital sinus in mice orplexus in rats with a sterile hematocrit capillary tube or Pasteur pipette. Sterile tubes are recommended tohelp avoid periorbital infection and potential long-term damage to the eye.
What does retro-orbital bleeding stand for?
Technique. Also referred to as peri-orbital, posterior-orbital and orbital venous sinus bleeding. Retro-orbital bleeding should be performed as a terminal procedure. It should only be used with recovery in rare circumstances with exceptional scientific justification (e.g. where a large blood volume is necessary or where peripheral veins are used…
What do we know about blood sampling techniques in rats?
We compared the behaviours of rats, and measured various blood parameters, after three blood sampling techniques: orbital puncture while they were under diethyl-ether anaesthesia, blood collection by tail vein puncture under O2-N2O-halothane anaesthesia and puncture of the saphenous vein without anaesthesia.
