What does CAG promoter stand for?
What does CAG promoter stand for?
CMV enhancer
Actually, CAG stands for CMV enhancer, chicken beta-Actin promoter and rabbit beta-Globin splice acceptor site, a combined fusion for very efficient expression in eukaryote cells.
How does the CAG promoter work?
The CAG promoter is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian expression vectors. In addition to the CMV immediate early enhancer, the intron of the chicken beta actin gene contains an enhancer element, which is highly conserved among vertebrates. …
Is CAG promoter constitutive?
Some other constitutive promoters, including human β-actin (hACTB), human elongation factor-1α (hEF-1α), and cytomegalovirus early enhancer/chicken β-actin (CAG) promoters, have shown their merits in sustaining stable gene expression for long periods of time [4]–[8], [12]–[17].
How big is the CAG promoter?
Plasmid: CAG promoter
| Analyze: | Sequence |
|---|---|
| Size: | 584 |
| Notes: | Hybrid construct consisting of the cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter. |
| Stable: | Unspecified |
| Constitutive: | Unspecified |
What is pCAG plasmid?
pCAG-GBP1-10gly-Gal4DBD is a mammalian expression vector designed to express the DBD portion of a GFP-dependent transcription factor. The SV40 ori sequence enables episomal replication of the plasmid in cells expressing the SV40 large-T antigen (e.g., 293T and COS-7 cells).
What is SV40 promoter?
The early promoter of the simian virus 40 (SV40) has been used as a model eukaryotic promoter for the study of DNA sequence elements and cellular factors that are involved in transcriptional control and initiation. Some of these elements are present in cellular genes, and may exhibit tissue-specificity in their action.
What is PCAG?
Post Clearance Audit Group (PCAG) – shall refer to an office created under Executive Order No. 160 s. 2003, as amended by Executive Order No. 46, s. 2017, mandated to conduct audit of importers, locators, and all parties engaged in customs clearance and processing.
