Do macrophages produce M-CSF?
Do macrophages produce M-CSF?
The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which causes hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection.
What is the difference between M-CSF and GM-CSF?
M-CSF exhibits a mostly homeostatic expression pattern, whereas GM-CSF is a product of cells activated during inflammatory or pathologic conditions. Accordingly, M-CSF regulates the numbers of various tissue macrophage and monocyte populations without altering their “activation” status.
What is the purpose of macrophages in the CSF?
Diverse functions of macrophages contribute to the regulation of organogenesis. As potent effector cells, macrophages produce a range of growth factors that stimulate angiogenesis, induce apoptosis and regulate tissue morphogenesis.
Where do macrophages differentiate?
Macrophage differentiation from monocytes occurs in the tissue in concomitance with the acquisition of a functional phenotype that depends on microenvironmental signals, thereby accounting for the many and apparently opposed macrophage functions.
What are macrophages derived from?
Macrophages originate from blood monocytes that leave the circulation to differentiate in different tissues. There is a substantial heterogeneity among each macrophage population, which most probably reflects the required level of specialisation within the environment of any given tissue.
What is the difference between a monocyte and a macrophage?
Monocytes are the largest type of white blood cells and play an important role in the adaptive immunity process. Macrophages are monocytes that have migrated from the bloodstream into any tissue in the body.
How does M-CSF affect macrophages and monocytes?
M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage.
How do you culture human monocytes with macrophage colony-stimulating factor?
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. For initiation of experiments, fresh or frozen monocytes are cultured in flasks for 1 week with M-CSF to induce their differentiation into macrophages.
How do you make macrophages from frozen monocytes?
For initiation of experiments, fresh or frozen monocytes are cultured in flasks for 1 week with M-CSF to induce their differentiation into macrophages. Then, the macrophages can be harvested and seeded into culture wells at required cell densities for carrying out experiments.
Is it possible to activate monocytes with MCSF?
It is long known that adhesion itself is capable of inducing monocyte activation and differentiation. You may use M-CSF to accelerate the process, but it is not mandatory. However, depending on the cytokines you use, the differentiation will give rise to distinct macrophage subtypes, as stated by others.
