How do you use feulgen stain?
How do you use feulgen stain?
In a routine Feulgen staining technique, slides are immersed in 5 mol/L HCl for 15 minutes, rinsed with distilled water for 3 minutes, stained with Schiff’s reagent for 90 minutes, washed for 10 minutes and finally stained with 1% light green for 15 minutes (7-8).
Why feulgen stain is used for DNA only?
The Feulgen technique selectively stains DNA, and under controlled conditions, can be used for the photometric determination of DNA content. RNA is not hydrolyzed by the HCl treatment and, thus, the reaction is DNA-specific.
What purpose does the methyl green Pyronin Y stain serve?
meth·yl green-·py·ro·nin stain a staining method useful for identification of plasma cells which are intensely pyroninophilic; a mixture of a green and a red dye that has the property of staining highly polymerized nucleic acid (DNA) green and low molecular weight nucleic acids (RNA) red.
Is RNA present in Plasmalemma?
The correct answer is (D) Plasmalemma. Ribonucleic acid (RNA) is the most important molecule in cell biology. RNA is absent in plasmalemma. Plasma lemma is the inner layer of the cell wall and it comprises two membranes separated by an opaque zone.
Which of the following stain is used for DNA and RNA staining?
SYBR Gold dye can be used to stain double or single-stranded DNA or to stain RNA.
Which chemical substances in the cells can be detected by staining the cells with methyl green and Pyronin?
The combination of methyl green with pyronin can differentiate between RNA and DNA as the DNA appears green and RNA red.
What is the Feulgen test?
The Feulgen test is not only specific for the localisation of DNA in chromosomes, but at the same time the intensity of the reaction may be considered to be an index of the amount of DNA present in the cell.
What is the Feulgen technique for DNA staining?
FEULGEN STAINING PROTOCOL. The Feulgen technique selectively stains DNA, and under controlled conditions, can be used for the photometric determination of DNA content. The reaction consits of two steps. Fixed material is treated for 8-10 min with 1N HCl in a water bath or oven at 60°C. Afterwards, the material is immediately transferred…
How do you measure DNA ploidy with Feulgen reaction?
Isolated intact single cells are by far the most commonly used targets to determine DNA ploidy with the Feulgen reaction. Quantifying the stained Feulegen reaction was originally achieved using cytophotometry. Later it was found that DNA ploidy could also be measured by a microscope cytofluorometer.
What did Robert Feulgen discover?
Feulgen, Robert, German nucleic acid biochemist and cytochemist, 1884-1955, first to detect DNA in cells by a specific cytochemical test. Feulgen reaction- DNA staining reaction. Feulgen test Kasten fluorescent Feuglen stain- see under Kasten
