How long does it take for Caco-2 cells to adhere?
How long does it take for Caco-2 cells to adhere?
21 days
Although short time protocols for obtaining a polarized Caco-2 cell monolayer has been published, the standard and most reliable procedure requires 21 days of culture to obtain a polarized monolayer with full expression of tight junctions and other intercellular contacts.
Why are Caco-2 cells used?
Caco-2 is an immortalized cell line of human colorectal adenocarcinoma cells. It is primarily used as a model of the intestinal epithelial barrier. In culture, Caco-2 cells spontaneously differentiate into a heterogeneous mixture of intestinal epithelial cells.
How do you differentiate Caco-2 cells?
Standard methods used to differentiate Caco-2 cells involve seeding them on culture inserts fitted with polycarbonate filters, and allowing spontaneous differentiation to proceed for two to three weeks in culture medium containing 10% or 20% foetal bovine serum (FBS) in both the apical (AP) and basolateral (BL) …
What are calu 3 cells?
Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene. They express wildtype EGFR and mutant K-Ras (G13D). In addition, they harbor mutations in TP53 and CDKN2A genes.
What is a good Caco-2 permeability?
Most drugs are not especially permeable through Caco-2 cells, with the median Papp value being some 16 ⋅ 10−6 cm s−1. This value is considerably lower than those (1,310 and 230 ⋅ 10−6 cm s−1) recently used in some simulations that purported to show that Papp values were too great to be transporter-mediated only.
What is a major limitation to the Caco-2 monolayer permeability assay?
in 2007 regarding the use of Caco-2 monolayers in permeability assays [15]. The major limitation of this reference protocol is its very low throughput due to the long culture period (at least 21 days), although this has been shown to be required to obtain a fully differentiated and confluent monolayer [16,17].
Do Caco-2 cells produce mucus?
However, Caco-2 cells are mostly reported as non/low-mucus producing cells and do not or hardly express mucin-related genes, such as MUC-2 and MUC-625.
What does Caco-2 stand for?
Freebase. Caco-2. The Caco-2 cell line is a continuous line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh.
What are NHBE cells?
Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. All Cryopreserved human bronchial epithelial cells are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi.
What is Caco-2 permeability assay?
Cyprotex’s Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarised Caco-2 cell monolayers and from which the data generated can be used to predict in vivo absorption of drugs. The Caco-2 cell line is derived from a human colon carcinoma.
What are ht29 cells?
HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. These cells are sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which are standard treatment options for colorectal cancer.
