Is Alexa Fluor 488 GFP?
Is Alexa Fluor 488 GFP?
GFP Alexa Fluor® 488-conjugated Antibody Summary Detects recombinant GFPuv and eGFP in Western blots. Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
What does GFP bind?
Biologists use GFP as a marker protein. GFP can attach to and mark another protein with fluorescence, enabling scientists to see the presence of the particular protein in an organic structure. Gfp refers to the gene that produces green fluorescent protein.
Is GFP and FITC the same?
Green Fluorescent Protein (GFP) Antibody (FITC) is a Rabbit Polyclonal antibody conjugated to FITC against GFP.
What makes GFP unique?
GFP, on the other hand, has all of its own light handling machinery built in, constructed using only amino acids. It has a special sequence of three amino acids: serine-tyrosine-glycine (sometimes, the serine is replaced by the similar threonine).
What is anti GFP?
Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.
At what wavelength does GFP fluorescence?
The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79.
What is a GFP Axolotl?
Tanaka of Max Planck Institute has created a transgenic GFP axolotl, a half-foot long salamander that dwells in the Aztec canals of Mexico City. In order to monitor the axolotl’s limb regeneration process, the Max Planck scientists have inserted a green fluorescent protein into a mutant axolotl that lacks skin pigment.
How does fluorescence work?
fluorescence, emission of electromagnetic radiation, usually visible light, caused by excitation of atoms in a material, which then reemit almost immediately (within about 10−8 seconds). The initial excitation is usually caused by absorption of energy from incident radiation or particles, such as X-rays or electrons.
What is the difference between GFP and EGFP?
The key difference between GFP and EGFP is that the GFP is a wild-type protein incorporated in the molecular cloning of non-mammalian cells while the EGFP is an improved or engineered type of GFP that can be used on mammalian cells.
What causes fluorescence in GFP?
victoria, GFP fluorescence occurs when aequorin interacts with Ca2+ ions, inducing a blue glow. Some of this luminescent energy is transferred to the GFP, shifting the overall color towards green.
How many kDa is GFP?
27 kDa
The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa.
Can antibodies bind to other antibodies?
What is a secondary antibody? A secondary antibody is one that recognizes an antibody or antibody domain from a different species. Secondary antibodies are used to bind primary antibodies (specific for a protein of interest (antigen)) in many different experimental schemes.
Can immunofluorescein conjugated anti-GFP be used to detect insert GFP?
Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic ( E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.
What does GFP stand for?
Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm. We offer a series of GFP Invitrogen CellLight…
What is GFP used for in microbiology?
GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
What type of protein is gtgfp?
GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors.
