Is MCF10A cancer cell line?

Is MCF10A cancer cell line?

The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures.

What are MCF10A cells?

MCF10A cells are widely used non-malignant breast epithelial cells. They exhibit some features of normal breast epithelium, including lack of anchorage-independent growth and dependence on growth factors and hormones for proliferation and survival [6]. They can form acinar structures in 3D culture [37].

What are HMEC cells?

Lonza’s Human Mammary Epithelial Cells (HMEC) are isolated from adult female breast tissue. The cells have been primarily utilized to understand the formation of breast cancer.

What are t47d cells?

T-47D is a human breast cancer cell line commonly used in biomedical research involving the hormonal expression of cancer cells.

Is MCF10A triple negative?

2.1. ]; MCF-7, MCF10A, SKBr3, and MDA-MB-231 were purchased from the American Type Culture Collection (ATCC). MDA-MB-231 cells are characterized as triple-negative/basal-B mammary carcinoma and SKBr3 are HER2+ mammary carcinoma.

What is mcf7 cell line?

MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27].

How do mammary glands develop?

Mammary gland function is regulated by hormones. At puberty, increasing levels of estrogen stimulate the development of glandular tissue in the female breast. Estrogen also causes the breast to increase in size through the accumulation of adipose tissue. Progesterone stimulates the development of the duct system.

What type of cells are mammary cells?

The mammary epithelium consists of two differentiated cell types organized into two cell layers, an inner layer of luminal epithelial and an outer layer of myoepithelial cells in direct contact with the basement membrane (Fig. 1a).

What is bt474 cell line?

The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast. Age 60 years Ethnicity White Gender Female Karyotype. The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range.

What is MCF7 cell line?

Is MCF-7 invasive?

Though MCF7 is categorized as poorly invasive, its migration in the 2D surface is found to be comparable to the highly invasive MDA MB 231 in the current study.

Are MCF-7 cells HER2 positive?

MCF-7 breast cancer cells stably overexpressing ERbeta1 exhibited elevated >5-fold HER2 mRNA levels and elevated >3-fold HER2 protein levels even in the absence of estradiol.

Are MCF10A cells Mammosphere-forming cells?

When grown in suspension culture, MCF10A cells showed low mammosphere-forming ability. Cells in mammospheres and 3D culture expressed both luminal and basal markers.

What do we know about stress fiber formation in MCF10A cells?

Around 50% of cells displayed strong staining of CK5, whereas around 10% of cells expressed moderate levels of CK17. Phalloidin staining (F-actin) indicated stress fiber formation in MCF10A cells. Surprisingly, MCF10A cells exhibited completely negative staining of P63 and less than 1% of cells were positive for CK14.

Is the acinar structure formed by MCF10A cells unique in breast tissue?

Surprisingly, the acinar structure formed by MCF10A cells in 3D culture was positive for both basal markers and the milk proteins β-casein and α-lactalbumin. MCF10A cells exhibit a unique differentiated phenotype in 3D culture which may not exist or be rare in normal human breast tissue.

What is the MCF10A model used for?

The 3D MCF10A model provides a useful tool for dissecting cell-cell interactions in mammary gland development, as well as for studying the effects of microenvironment on mammary cell function and the effects of different genetic or non-genetic modifications on mammary cell transformation.