What are Hep-2 cell lines?
What are Hep-2 cell lines?
Hep-2/Hep2 cells are invaluable for researchers engaged in the analysis of autoantibodies, and they are currently one of the most common substrates for antinuclear antibody detection by immunofluorescence [6,7]. The Hep-2 cell line was first specifically described by H.W. Toolan in 1954 [8].
Where do Hep-2 cells come from?
Int 407 cells were originally cultured from human embryonic intestine and developed into their present epithelial phenotype after 60 passages in culture (Henle and Deinhardt, 1957). The HEp-2 cell line originated from tumours produced after injection of rats with epidermoid carcinoma tissue.
What are Hep 2c cells?
The HEp-2 cell line contains HeLa marker chromosomes and were derived via HeLa contamination. The cells are positive for keratin by immunoperoxidase staining. ATCC confirmed this cell line is positive for the presence of human papilloma viral DNA sequences via PCR.
What is HEp-2 blood test?
Summary: The presence of antinuclear antibodies (ANA) indicates the possibility of autoimmunity and the indirect immunofluorescence (IIF) assay on HEp-2 cells is the standard blood test (ANA-HEp-2) used to detect ANA.
What is ANA screen on HEp-2?
The presence of antinuclear antibodies (ANA) indicates the possibility of autoimmunity and the indirect immunofluorescence (IIF) assay on HEp-2 cells is the standard blood test (ANA-HEp-2) used to detect ANA.
What is KB cell line?
This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa.
How did John Hopkins treat Henrietta Lacks?
In 1951, Henrietta Lacks was diagnosed with cervical cancer and was treated at the segregated Johns Hopkins Hospital with radium tube inserts, a standard treatment at the time. As a matter of routine, samples of her cervix were removed without permission.
What is hepatitis 2 antibody?
The indirect immunofluorescence assay (IIFA) on HEp-2 cells is widely used for detection of antinuclear antibodies (ANA). The dichotomous outcome, negative or positive, is integrated in diagnostic and classification criteria for several systemic autoimmune diseases.
What does ANA positive HEp-2 mean?
A positive result on an ANA test means that antinuclear antibodies were found in your blood. You may get a positive result if: You have SLE (lupus). You have a different type of autoimmune disease. You have a viral infection.
