What does a spec 20 measure?

What does a spec 20 measure?

The Spectronic 20, often called the Spec 20, is a spectrophotometer. A spectrophotometer measures the intensity of a light beam before and after it passes through a sample and compares these two intensities. The Spec 20 reports two types of measurements: percent transmittance and absorbance.

What is the readout device of spectronic 20d?

The Bausch & Lomb Spectronic 20 colorimeter uses a diffraction grating monochromator combined with a system for the detection, amplification, and measurement of light wavelengths in the 340 nm to 950 nm range.

What spectronic 21?

Specifications & Description SPECTSPECTRONIC 21 Digital Model with UV Capabilities features linear digital readouts in % T, A, and concentration; push-button decimal selection; and an RS-232 interface port. Three-position sensitivity switch and the 100% T/zero A control let you select desired instrument gain setting.

Does the volume in the cuvette affect the reading?

The concentration of a bacterial culture is typically determined by measuring the absorbance of a sample at a wavelength of 600 nm, commonly called the “OD600.” The larger sample volume of a macro cuvette allows for heterogeneous samples such as this to provide a more reliable reading.

What is AMAX chemistry?

Illustrated Glossary of Organic Chemistry – Lambda max. Lambda max (λmax): The wavelength at which a substance has its strongest photon absorption (highest point along the spectrum’s y-axis). This ultraviolet-visible spectrum for lycopene has λmax = 471 nm.

How do you zero a spectrophotometer?

This allows you to set a “0” so that your absorbance readings will have a basis to be compared to. Zero (blank) the SmartSpec 3000 by placing a cuvette containing only media (NO BACTERIA) into the cuvette chamber and pressing Read Blank. You can re-zero the instrument at any time during use.

How much should you put in a cuvette?

Fill the cuvette about 3/4 full of the solution you wish to test. Wipe the outside of the cuvette with a lint-free, soft tissue (a Shurwipe or and Accuwipe) to remove any moisture or fingerprints from the outside surface.

What happens if you don’t wipe cuvette?

Wipe the cuvette with a Kimwipe to remove any liquid and fingerprints on the outside of the cuvette. Both of these will interfere with light transmission and will cause erroneous readings. Scratches on the cuvette can lead to erroneous measurements.

How do you use a Spec 20 Cuvette?

The cuvettes for use in the Spec 20 resemble small test tubes. Each cuvette is markded so that it can be positioned properly in the sample holder. The mark is at the top of the cuvette and must be positioned toward teh front of the spectrophotometer when taking measurements.

What is the correct path length for my Cuvette?

Basically, depending on how you put the cuvette in your spectrophotometer will determine which path length is used. If the light beam enters the front window, then you have a 10 mm path length cuvette. If you rotate the cuvette 90 degrees you have a 2 mm path length. We have a few different cuvettes with dual-path lengths available such as:

What is the MEASURING volume of a macro Cuvette?

The measuring volume of a macro cuvette is greater than 3.5 mL (7 – 35mL). A standard volume cuvette holds a measuring volume of 3.5 mL. A semi micro cuvette volume holds samples of 0.35 mL – 1.7 mL. Among these types of short path length cuvettes may require cuvette mounts or spacers.

What are the different types of cuvettes?

Cuvette Materials 1 Optical Glass Cuvettes. Optical glass cuvettes are suitable for transmission wavelengths from 340nm to 2,500nm (Visible Spectrum). 2 ES Quartz Cuvettes. ES quartz cuvettes for UV light are suitable for transmission wavelengths from 190nm to 2,500nm (UV Spectrum). 3 IR Quartz Cuvettes.