What does Qiagen protease do?
What does Qiagen protease do?
QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.
What is proteinase K used for?
Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.
How does Qiagen DNA extraction work?
QIAamp DNA technology yields genomic, mitochondrial, bacterial, parasite or viral DNA from human tissue samples ready to use in PCR and blotting procedures. During the QIAamp DNA purification procedure, DNA binds specifically to the QIAamp MinElute or QIAamp silica-gel membrane while contaminants pass through.
How do you store proteinase K solution?
Storage conditions Proteinase K, 20 mg/mL AM2546: 1.25 mL AM2548: 5 × 1.25 mL Store at –20°C. Do not store in a frost-free freezer. This product is guaranteed for 6 months from the date of receipt, if properly stored.
What is the difference between protease and proteinase K?
The key difference between proteinase K and protease is that proteinase K is useful in digesting proteins and removing contamination from nucleic acid preparations, whereas protease is useful in biological functions such as digestion of ingested proteins, protein catabolism, and cell signaling.
Is proteinase K protease inhibitor?
Proteinase K is inhibited by serine protease inhibitors such as phenylmethylsulfonyl fluoride (PMSF), diisopropylfluorophosphate (DFP), or 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF).
How long is proteinase K good for?
1 year
QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.
Can you use too much proteinase K?
Proteinase K is a protein and it will constitute a trivial amount of the protein in the extract and will be removed in the Ph-CHCl3 extraction. The SDS might hang around if you use too much, especially if you ethanol precipitate (though I suspect SDS is quite ethanol-soluble).
What is the Qiagen miniprep procedure?
The QIAprep Miniprep procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt (1). The unique silica membrane used in the QIAprep Miniprep Kit completely replaces glass or silica slurries for plasmid DNA minipreps.
Where does proteinase cleave?
Proteinase K is able to digest hair (keratin), hence, the name “Proteinase K”. The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.
What is a unit of proteinase K?
Proteinase K from NEB is sold at 800 Units/ml ± 10% (indicated as a black line with error bars), whereas most other vendors sell Proteinase K as > 800 or > 600 Units/ml.
What is QIAGEN proteinase K?
QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album and is particularly suitable for short digestion times. It possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature.
How long is an aqueous solution of Proteinase K good for?
How long is an aqueous solution of Proteinase K from Qiagen good for if it is kept at room temperature? The proteinase K may be up to 4 years old. Everything online indicates that Proteinase K can be good for up to 2 years if at 4C or below, but there is really no information on how long it could really be good for.
Is proteinase K available through Promega helix?
This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab’s needs. Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity.
What is the pH of QIAGEN protease?
* pH 8.0, 50°C, 1.25 µg/ml protease, 15 min incubation. † Buffer G2 used in QIAGEN Genomic-tip procedures for DNA isolation from blood, cell cultures, and tissue.
