What is Lactophenol blue used for?
What is Lactophenol blue used for?
lactophenol cotton blue definition Lactophenol Blue Solution or Lactophenol cotton blue is a wet mounting medium and staining agent which is used for the preparation of microscopic slides of fungi for examination. Fungal elements are stained intensely blue.
Why we use Lactophenol cotton blue in fungal staining?
Phenol kills fungus. Lactic acid acts as a clearing agent and helps preserve the fungal structures. Cotton blue is an aniline dye that stains the chitin in the fungal cell walls which adds colour to the fungal preparation thereby enhancing and contrasting the structures.
What is Lactophenol cotton blue used for explain the method?
Lactophenol cotton blue wet mount preparation is commonly used for microscopic identification of fungi. It is formulated with lactophenol, which serves as the mounting fluid and the dye, cotton blue. Lactic acid preserves the fungal structure and clears the tissue while phenol acts as a disinfectant.
What are the four major components of Lactophenol cotton blue?
Lactophenol Cotton Blue (LPCB).
- Cotton blue (Aniline blue) 0.05 g.
- Phenol crystals (C6H5O4) 20 g.
- Glycerol 40 mL.
- Lactic acid (CH3CHOH COOH) 20 mL.
- Distilled water 20 mL.
What is the role of the phenol in Lactophenol cotton blue?
The preparation has three components: phenol, which will kill any live organisms; lactic acid which preserves fungal structures, and cotton blue which stains the chitin in the fungal cell walls.
What is the composition of lactophenol blue?
Lactophenol cotton blue (LCB) is a mixture of methyl blue, a histological stain, and lactophenol (a solution of phenol, lactic acid, and glycerol in water). It is used in wet-mount preparations for visualization of fungal structures, especially in medical mycology.
How does lactophenol aniline blue work?
Remel Lactophenol Aniline Blue is a stain recommended for use in wet mount preparations for microscopic examination of fungi. Phenol precipitates cytoplasmic proteins and inactivates enzyme systems within the fungal cell. Glycerol prevents drying, allowing for examination of the stain 18-24 hours after preparation.
What is the composition of Lactophenol blue?
How do you make lactophenol solution?
A preparation of 50ml Lactophenol cotton Blue staining solution is made up of:
- Distilled water 50ml.
- Cotton Blue (Aniline Blue) 0.125g.
- Phenol Crystals (C6H5O4) 50g.
- Glycerol 100ml.
- Lactic acid (CH3CHOH COOH) 50ml.
- 70% ethanol.
How does Lactophenol aniline blue work?
How do you stain fungal spores?
The optimal staining condition were as follows: fungal spores are pretreated with ultrasound at 495 W for 5 min, then stained by adding 10 μL of SG (100×) and 30 mM EDTA per 500 μL water sample for 20 min at 35 °C in dark. The fungal spore quantification by FCM is a reliable and reproducible counting method.
What are the composition of lactophenol?
How do you use lactophenol cotton blue to stain fungi?
The lactophenol cotton blue solution acts as a mounting solution as well as a staining agent. The stain will give the fungi a blue-colored appearance of the fungal spores and structures, such as hyphae. A preparation of 50ml Lactophenol cotton Blue staining solution is made up of:
What is lactophenol cotton blue (LPCB) stain?
The Lactophenol Cotton Blue (LPCB) wet mount is most widely used method for the staining and observation of fungi. LPCB is a stain used for making semi-permanent microscopic preparation of fungi.
What is lactophenol stain used for?
Lactophenol cotton blue stain is used to identify fungi grown on culture from any clinical material. Principle of Lactophenol cotton blue stain The stain is used to stain fungus grown on ‘slide culture technique’ or culture media, and the preparation is examined under a bright field microscope.
How to identify Scopulariopsis species using lactophenol?
Figure: First: Scopulariopsis species on a lactophenol cotton blue stain. Image Source: stylish_streaking and Senthil Prabhu. It can only be used as a presumptive identification method of fungi which should be followed up with other diagnostic tools such as biochemical and cultural examination.
