What is MALDI-TOF mass spectrometry used for?
What is MALDI-TOF mass spectrometry used for?
Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory.
What is MALDI-TOF mass spectrometry in microbiology?
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) is a powerful analytical mass spectrometry technique that has generated numerous diagnostic and clinical applications, especially for the identification of microorganisms for medical diagnosis.
How does MALDI-TOF MS identify bacteria?
Although used since the 1980s in biochemistry, MALDI-TOF MS only reached clinical microbiology laboratories in 2010. This technique quickly identifies bacterial species by determining the molecular masses of proteins, many of which are ribosomal proteins.
What type of test is Maldi-Tof?
MALDI-TOF MS is an analytical technique in which particles are ionized, separated according to their mass-to-charge ratio, and measured by determining the time it takes for the ions to travel to a detector at the end of a time-of-flight tube.
What types of sample are suitable for Maldi-TOF mass analysis?
Samples for MALDI analysis may be supplied either in solution or lyophilised. Ideally samples should be resuspended in an aqueous solution of 50% (v/v) acetonitrile, 0.1% (v/v) trifluoroacetic acid using ultra high purity water. If any other solution is used, BioCentre staff must be supplied with comprehensive details.
What are the advantages and disadvantages of Maldi-Tof?
Table 1
| Detection method | Advantages | Disadvantages |
|---|---|---|
| MALDI-TOF MS | • Fast • Accurate • Less expensive than molecular and immunological-based detection methods • Trained laboratory personnel not required | • High initial cost of the MALDI-TOF equipment |
Which analyzer is more significant TOF or quadrupole?
The advantages of time-of-flight mass analyzers compared to quadrupoles are clear. Samples can be measured faster and with no spectral skewing. For the same mass range, a TOF analyzer will measure each ion more sensitively.
Is Maldi-Tof accurate?
The MALDI-TOF MS correctly identified 92% of the M. tuberculosis isolates (95% CI of 0.87 to 0.96), and 68% of M. The identifying accuracy rate of bioMérieuxVitek MS was slight higher than Bruker MALDI Biotyper (75% vs 72%). However, opposite results were obtained in identifications of M.
How much sample do you need for MALDI?
A: You can sonicate it for 10 minutes in a large beaker with 50% methanol and roughly 5% acetic acid. Q: How much sample do I need to perform MALDI-TOF analysis? A: You need approximately 10 uL of sample. Less can be used because each spot requires approximately 1 uL of sample and 1 uL of matrix.
What information does mass spectrometry provide?
Mass spectrometry. A mass spectrum is a plot of the ion signal as a function of the mass-to-charge ratio. These spectra are used to determine the elemental or isotopic signature of a sample, the masses of particles and of molecules, and to elucidate the chemical structures of molecules and other chemical compounds .
What is a mass spectrometer used for?
Mass spectrometry (MS) is an analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio. In simpler terms, a mass spectrum measures the masses within a sample. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures.
What does the mass spectrometer do?
The mass spectrometer is an instrument which can measure the masses and relative concentrations of atoms and molecules. It makes use of the basic magnetic force on a moving charged particle.
How does mass spectrometry testing work?
Because mass spectroscopy measures the mass of charged particles, only ions will be detected, and neutral molecules will not be seen. Ions are created by giving electrons to a molecule (producing a negatively charged ion) or taking electrons away from a molecule (producing a positively charged ion).
