What is the use of ITS2?
What is the use of ITS2?
ITS2 sequences have a relatively high divergence rate; thus, it can be used as a complementary locus to CO1 for identification of animal species. Recently, ITS2 region has been found to vary in primary sequences and secondary structures in a way that correlates highly with taxonomic classification.
What is ITS2 gene?
In bacteria and archaea, there is a single ITS, located between the 16S and 23S rRNA genes. ITS1 corresponds to the ITS in bacteria and archaea, while ITS2 originated as an insertion that interrupted the ancestral 23S rRNA gene.
What is ITS2 sequencing?
The internal transcribed spacer 2 (ITS2) has been used as a phylogenetic marker for more than two decades. It also provides several tools to process your own ITS2 sequences, including annotation, structural prediction, motif detection and BLAST14 search on the combined sequence-structure information.
What is the difference between ITS1 and ITS2?
The key difference between ITS1 and ITS2 is that ITS1 is a spacer DNA located between 18S and 5.8S rRNA genes in eukaryotes, while ITS2 is a spacer DNA located between 5.8S and 28S rRNA genes in eukaryotes. Therefore, ITS1 and ITS2 are two spacer DNA found between rRNA genes in eukaryotes.
What is ITS1 and ITS4?
ITS1 and ITS4 are general primers that amplifies the Internal Transcribed Spacer region for identification purpose. You can easily find a journal that provides the sequences for these primers: https://benthamopen.com/FULLTEXT/TOBIOTJ-14-70. Cite.
How long is the ITS2 region?
Additionally, the significantly smaller size of ITS2 (average length of approximately 233 bp) compared with that of ITS (average length of approximately 634 bp) makes ITS2 a better candidate for barcoding studies. Identification efficiency of ITS and ITS2 by using BLAST.
Is ribosome A DNA?
What Is Ribosomal DNA? Ribosomal DNA, or ribosomal deoxyribonucleic acid (rDNA), is the DNA that encodes ribosomal proteins that form ribosomes. This rDNA makes up a relatively small portion of human DNA, but its role is crucial for several processes.
What is matK gene?
Maturase K (matK) is a plant plastidial gene. The protein it encodes is an organelle intron maturase, a protein that splices Group II introns. It is essential for in vivo splicing of Group II introns.
What is the most promising DNA barcode for plants?
ycf1b
For the well-sampled representative plant groups, ycf1b generally performed better than any of the matK, rbcL and trnH-psbA. We concluded that ycf1a or ycf1b is the most variable plastid genome region and can serve as a core barcode of land plants.
Why do we use 16S?
Since 16S rRNA gene is conserved in bacteria, and contain hypervariable regions that can provide species-specific signature sequences, 16S rRNA sequencing is widely used in identification of bacteria and phylogenetic studies. 16S rRNA sequencing is featured by fast speed, cost-efficiency, and high-precision.
Is RNA the same as rDNA?
Ribosomal DNA (rDNA) is a DNA sequence that codes for ribosomal RNA.
What is the rbcL gene?
The rbcL gene is the barcode DNA for plant species. J.R. Abbott 23676 (FLAS) ribulose-1,5-bisphosphate carboxylase large subunit (rbcL) gene, but phylogenetically the precise position of sample could not found because the limitation of rbcL gene.
