What protein does beta-lactamase code for?
What protein does beta-lactamase code for?
Beta-lactamase provides antibiotic resistance by breaking the antibiotics’ structure. These antibiotics all have a common element in their molecular structure: a four-atom ring known as a β-lactam….Beta-lactamase Edit Wikipedia article.
| Available protein structures:[show] | |
|---|---|
| PDBsum | structure summary |
How do you determine the amino acid sequence of a gene?
How to: Find transcript sequences for a gene
- Search the Gene database with the gene name, symbol.
- Click on the desired gene.
- Click on Reference Sequences in the Table of Contents at the upper right of the gene record.
What type of protein is a beta-lactamase?
Beta-lactamase is a type of enzyme (EC 3.5. 2.6) produced by some bacteria that is responsible for their resistance to beta-lactam antibiotics like penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam.
What does bla gene stand for?
bla — gene that encodes β-lactamase, an enzyme that breaks down the antibiotic ampicillin; transformants expressing the bla gene can be selected by placing ampicillin in the growth medium.
Is beta-lactamase a protein?
Beta-lactamase provides antibiotic resistance by breaking the antibiotics’ structure. These antibiotics all have a common element in their molecular structure: a four-atom ring known as a β-lactam….Beta-lactamase.
| Metallo-beta-lactamase | |
|---|---|
| Pfam clan | CL0381 |
| InterPro | IPR001279 |
| showAvailable protein structures: |
How do you determine protein sequence?
The two major direct methods of protein sequencing are mass spectrometry and Edman degradation using a protein sequenator (sequencer). Mass spectrometry methods are now the most widely used for protein sequencing and identification but Edman degradation remains a valuable tool for characterizing a protein’s N-terminus.
How do you determine the amino acid sequence of a protein?
There are two main methods used to find the amino acid sequences of proteins. Mass spectrometry is the most common method in use today because of its ease of use. Edman degradation using a protein sequenator is the second method, which is most useful if the N-terminus of a protein needs to be characterized.
What protein does bla code for?
The bla gene encodes a 332-residue protein (Mr, 35,218), similar to other class A beta-lactamases produced by actinomycetes.
What is TEM type beta lactamase?
TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime.
Do beta-lactamases have a polyphyletic origin?
The present evidence suggests that the beta-lactamases have a polyphyletic origin. All the beta-lactamases of currently known amino acid sequence belong to one homology group, here called class A enzymes.
Is class B lactamase a Class A enzyme?
Class B consists of the mechanistically distinct Bacillus cereus beta-lactamase II, which preliminary partial sequence analysis suggests to be structurally unrelated to the class A enzymes.
Do beta-lactamases inactivate penicillins and cephalosporins in Gram-positive bacteria?
R P Ambler PMID: 6109327 DOI: 10.1098/rstb.1980.0049 Abstract The beta-lactamases are widely distributed in both Gram-positive and Gram-negative bacteria. They all inactivate penicillins and cephalosporins by opening the beta-lactam ring.
