Which method is best for creatinine test?
Which method is best for creatinine test?
The Jaffe and enzymatic methods are the two most common methods for measuring serum creatinine. The Jaffe method is less expensive than the enzymatic method but is also more susceptible to interferences. Interferences can lead to misdiagnosis but interferences may vary by patient population.
What are the limitations of Jaffe’s method?
The Jaffe method is subject to bias due to interfering substances (loss of analytical specificity). The risk of misclassification is greatest at the 60 ml/min/1.73m2 decision limit; however, the risk of misclassification due to bias is much less than the risk of misclassification due to biological variation.
What is the linearity of the Jaffe reaction method to measure creatinine?
The Jaffe creatinine method has a limit of quantitation (LOQ) of 0.2 mg/dL, a limit of linearity (LOL) of 38.7 mg/dL and an extended measuring range of 0.2‐387 mg/dL (with auto rerun), according to the manufacturer.
How is creatinine calculated?
Creatinine Clearance Calculator Creatinine Clearance = [[140 – age(yr)]*weight(kg)]/[72*serum Cr(mg/dL)] (multiply by 0.85 for women). The results you receive from this tool are for informational purposes only and should not be the basis of your medical decision making.
Which method estimates blood creatinine level most accurately?
Enzymatic creatinine assay
Enzymatic creatinine assay. Widely accepted as one of the most accurate routine methods available at present and data in Table 3 indicates that this method produces results for patient samples that agree closely with IDMS.
Is urea and creatinine the same thing?
Urea and creatinine are nitrogenous end products of metabolism. Urea is the primary metabolite derived from dietary protein and tissue protein turnover. Creatinine is the product of muscle creatine catabolism.
Why are creatinine levels checked?
A creatinine test is used to see if your kidneys are working normally. It’s often ordered along with another kidney test called blood urea nitrogen (BUN) or as part of a comprehensive metabolic panel (CMP). A CMP is a group of tests that provide information about different organs and systems in the body.
What is modified Jaffe?
Principle of Modified Jaffe’s kinetic method:- Creatinine in the specimen reacts with alkaline picrate in an alkaline medium provided by sodium hydroxide to create a reddish colored creatinine picrate complex.
What is a normal CRCL?
Normal Results Normal values are: Male: 97 to 137 mL/min (1.65 to 2.33 mL/s). Female: 88 to 128 mL/min (1.496 to 2.18 mL/s).
How long has the Jaffe method been used?
The method is still in use 125 years following the discovery of the principle, which is a fact unparalleled in clinical chemistry. Despite the advent of the enzymatic creatinine analysis, the Jaffe method is still popular due to its simplicity and low cost.
Why is it called the Jaffe principle?
Among kidney researchers, Jaffe is well known for having given his name to an analytical principle for assaying creatinine in human body fluids. By 1875, it was acknowledged that adding saturated picric acid solutions to human urine produced crystals, which were then attributed to uric acid [4].
What is Jaffe’s reaction for creatinine?
Creatinine in serum or urine is determined by Jaffe’s reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium. After allowing an incubation time of 15 min at room temperature for color development the color is measured at 520 nm.
How long does it take for Jaffe reaction to develop?
The reaction time variation among Jaffe recipes was huge from the beginning. Folin initially gave a time limit of 5–10 min for the development of the Jaffe reaction. Later, he restricted the time limit to 5 min and this period was later adopted by most workers.
