How do you study Unculturable bacteria? The only way to determine the presence of unculturable bacteria is by a process called whole genome sequencing. What this does is take a sample of (say) seawater and sequence all the DNA present inside it. Some of the DNA will be from culturable strains and these can be […]
How do you identify interacting partners of a protein? Fluorescence resonance energy transfer (FRET) is a common technique when observing the interactions of different proteins. Applied in vivo, FRET has been used to detect the location and interactions of genes and cellular structures including integrins and membrane proteins. How do two proteins interact with each […]
Is Hakone Free Pass included in JR pass? If you have a JR Rail pass, take the Shinkansen to Odawara. At Odawara, you can by a Hakone Freepass that will cover your travels in the area. The Odakyu Line also runs directly from Shinjuku to Hakone-Yumoto but is not covered by the JR Rail pass. […]
How do you babysit a bratty kid? The four steps to babysitting difficult children: Step 1: Maintain Structure & Routine. Step 2: Maintain Authority. Step 3: Maintain a Fun Atmosphere. Step 4: Maintain a Positive Attitude. To be clear, this is not an article discussing the care of children that are difficult because they’re not […]
Where did the Grateful Dead play in Chicago? Soldier Field Today marks 25 years since the Grateful Dead performed their final show. The July 9, 1995 concert at Soldier Field in Chicago was the last of that year’s summer tour, which was plagued by numerous incidents that led to it being known as the “Tour […]
How do I find my railroad crossing number? Note: The DOT number is a six digit number with an alpha character at the end (Ex.: 999 999A) and is found on the blue sign mounted on the crossbuck post for a passive crossing: it may be found on either the signal mast and/or signal cabin. […]
What are Rail anchors? Rail anchors, also called anticreepers, are spring steel clips that attach to the underside of the rail baseplate and bear against the sides of the sleepers to prevent longitudinal movement of the rail, either from changes in temperature or through vibration. Which is better wedge anchor or sleeve anchor? Because of […]
Does Sprite free have caffeine? Sprite and Fresca soda are also caffeine-free. Enjoy these popular caffeine-free drinks: Caffeine-Free Coca-Cola, Caffeine-Free Diet Coke and Caffeine-Free Coca-Cola Zero Sugar. Sprite and Sprite Zero. Does Sprite Zero ginger have caffeine? Sprite Zero Sugar Ginger is reinvention at its finest, taking a familiar flavor combination of lemon-lime and ginger, […]
How long should you use a rowing machine to lose weight? Workouts for Weight Loss When rowing for weight loss, workout consistency and duration are key. For maximum results, strive to complete 30–50 minutes of rowing five to six times per week. When will I see results from rowing? If you do this routine three […]
Is Fluke 87V good for electronics? It is ideal for troubleshooting electrical and electronic systems, built to handle complex signals and currents up to 20A. A True RMS meter, it is very accurate and has all the test ranges, including capacitance, an auto-touch feature and an excellent dual resolution, 20000-count display. When was the Fluke […]
