What does calf intestinal phosphatase do?
What does calf intestinal phosphatase do? Calf intestinal alkaline phosphatase (CIAP) catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vehicle DNA by removing phosphate groups from both 5´-termini. How do you turn off alkaline phosphatase? Alkaline Phosphatase […]