What does imidazole do in his tag?
What does imidazole do in his tag? A his-tag, or polyhistidine tag, is a string of histidine residues at either the N or C terminus of a recombinant protein. Imidazole competes with the his-tag for binding to the metal-charged resin and thus is used for elution of the protein from an IMAC column. What is […]