How do you determine if a protein is phosphorylated?
How do you determine if a protein is phosphorylated? Methods for Detecting Protein Phosphorylation Introduction. Kinase Activity Assays. Phospho-Specific Antibody Development. Western Blot. Enzyme-Linked Immunosorbent Assay (ELISA) Cell-Based ELISA. Intracellular Flow Cytometry and ICC/IHC. Mass Spectrometry. How do you know if a site is phosphorylated? Identification of Phosphorylation-site involves enriching phosphorylated peptides followed by mass […]