How is scFv generated?
How is scFv generated? There are two common strategies for generating scFvs: phage display or cloning of variable regions from mouse hybridoma. Generally, scFvs are engineered to contain an antigen-binding site by cloning heavy and light chain variable region genes (VH and VL) from hybridoma cells that secrete MAbs. Is scFv glycosylated? In humans, the […]