How do I make XL1 Blue competent cells?
How do I make XL1 Blue competent cells? Autoclave 100 ml LB- broth in a 1000 ml flask. Sterilize and pre-chill the 50-mL conical tubes. Prepare 30 ml of ice cold MgCl2-CaCl2 solution (80mM MgCl2, 20 mM CaCl2) Prepare ice cold 0.1 CaCl2. Pre-warm the LB plates. What are Electrocompetent cells? Electrocompetent cells are made […]