What is DNeasy blood and tissue kit?
What is DNeasy blood and tissue kit?
DNeasy Blood & Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial and pathogen) from a variety of sample sources including fresh or frozen animal tissues and cells, blood or bacteria.
What is DNeasy plant mini kit?
Qiagen’s DNeasy Plant DNA Extraction Mini Kit is a widely used kit in molecular biology labs for the routine extraction of genomic DNA from a range of plant material. Several protocols exist which use simple salt buffers such as Tris-EDTA and can generate higher yields of DNA.
What is genomic purification?
The purification is based on a set of solutions and a simple centrifugation procedure. Protocols are designed for an easy extraction and purification of genomic DNA from a wide range of samples, including whole blood, buffy coat, bone marrow, body fluids, buccal cells, tissues, mouse tails, etc.
What is in Al buffer?
The AL buffer contains the chaotropic agent guanidinium chloride. It promotes the lysis of the cell membrane, denaturation of proteins, DNA and other macromolecules. It also inactivates nucleases and promotes nucleic acid binding to pure silica material.
What is CTAB DNA extraction?
For every 100 mg of homogenized tissue add 500 µl of CTAB Buffer. Mix and thoroughly vortex. Place the tube in a 60°C water bath for 30 minutes. Centrifuge the homogenate for 5 minutes at 14,000 x g. Transfer supernatant to a new tube.
What does a QIAGEN miniprep do?
The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning.
How does a QIAGEN miniprep work?
The QIAprep Miniprep procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt (1). The unique silica membrane used in the QIAprep Miniprep Kit completely replaces glass or silica slurries for plasmid DNA minipreps.
What is the sample size of qiaamp DNA blood mini kit?
The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 µl, with a preparation time of 20–40 minutes. The typical yield from 200 µl healthy whole blood is 4–12 µg, with an elution volume of 50–200 µl.
What is the size of the purified DNA in QIAGEN genomic tip?
The purified DNA is sized up to 150 kb with an average size of 50–100 kb. When using the Blood & Cell Culture DNA Midi Kit, the purchase of the Genomic DNA Buffer Set (cat.no. 19060) is required for yeast and bacteria samples. Performance The QIAGEN Genomic-tip procedure is very gentle and results in negligible DNA shearing.
What can I do with purified DNA from the mini kit?
DNA purified using the QIAamp DNA Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, Southern blotting, SNP and STR genotyping and pharmacogenomic research.
What is the dneasy 96 blood & tissue kit?
For higher-throughput applications, the DNeasy 96 Blood & Tissue Kit enables simultaneous processing of 96 or 192 samples. DNeasy Blood & Tissue Kits provide high-quality DNA, ready to use in all downstream assays, including applications in: DNA yields. DNA was purified from 100 µl whole blood using the DNeasy Blood & Tissue Kit.
