What is a PCR test for mycoplasma?
What is a PCR test for mycoplasma?
The EZ-PCR™ Mycoplasma Detection Kit is a highly-sensitive and -specific PCR assay designed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures with a detection limit of 10 CFU/mL.
How do you test for mycoplasma contamination?
Mycoplasma contamination can be detected by polymerase chain reaction (PCR). PCR is easy, sensitive, specific, fast, reliable, efficient and costeffective. The PCR test is based on the detection of 16S rRNA molecules of the most common species of mycoplasma contaminating cell cultures.
Are ATCC cells tested for mycoplasma?
ATCC scientists use a proprietary PCR-based mycoplasma assay to test your cells. The assay quickly and reliably detects more than 60 species of mycoplasma, Acholeplasma, Spiroplasma, and Ureaplasma, including the top 8 species most likely to affect cell cultures: M. arginini, M.
How do you get rid of mycoplasma in chickens?
There is no cure for Mycoplasma and once a bird has it, they will have it for life and infect other birds. There are some antibiotics available through a veterinarian that may decrease the symptoms of Mycoplasma and speed up the bird’s recovery but no treatment will prevent it from spreading to other birds.
Who is mycoplasma standard?
The WHO Mycoplasma Standard (8293/13) is used globally to standardise nucleic acid technique-based assays for mycoplasma detection2. The Standard consists of Mycoplasma fermentans with a potency of 200,000 international units (IU) per ml.
What causes Mycoplasma in chickens?
It can also be spread from the droppings and feathers of infected birds and through dust and droplets in the air. Cages, coops and tools, such as shovels, used around birds with Mycoplasma can spread it to other birds, even after the sick birds are removed.
How does the lookout Mycoplasma PCR detection kit work?
The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.
What is microsart Mycoplasma qPCR?
Microsart ® Mycoplasma qPCR kits can be used with any qPCR cycler able to detect the fluorescent dyes FAM ® and ROX ®. Our clear, color-coded reagents guide the user through the short and simple protocol. Lyophilized reagents ensure consistent quality without the need for freezer storage.
How does the primer/probe system detect Mycoplasma?
The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin).
Are the PCR kits easy to use?
The kits are easy-to-use, very stable and contain all components required for a PCR test in a user-friendly format. Test Kit for Conventional PCR. Test Kit for Conventional PCR.
