What is EDC HCL?

What is EDC HCL?

1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, EDAC or EDCI) is a water-soluble carbodiimide usually handled as the hydrochloride. It is typically employed in the 4.0-6.0 pH range. It is generally used as a carboxyl activating agent for the coupling of primary amines to yield amide bonds.

How does EDC NHS chemistry work?

EDC, in conjunction with NHS allows, for 2-step coupling of two proteins without affecting the carboxyls of the second protein. First, EDC activates carboxyl groups and forms an amine reactive O-acylisourea intermediate that spontaneously reacts with primary amines to form an amide bond and an isourea by-product.

What is EDC crosslinking?

1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC or EDAC) is a zero-length crosslinking agent used to couple carboxyl groups to primary amines.

What is EDC NHS coupling reaction?

EDC/NHS coupling chemistry is commonly used to alter the surface chemistry of NPs with biological molecules to reduce their toxicity [31], [32]. EDC is a water-soluble cross-linker agent, which forms amide bonds between carboxyl and amine groups.

Is EDC soluble in acetone?

EDC and NHS are highly water and acetone soluble and should be no problem to be removed.

Is EDC soluble in water?

EDC has a high solubility in water (> then 200g/l) and is also soluble in a variety of organic solvents e.g. dichloromethane, tetrahydrofuran and dimethylformamide. Although EDC can be used for reactions in aqueous solution it is usually stored under dry conditions to prevent slow hydrolysis to the corresponding urea.

Does EDC react with water?

Can phosphate groups be crosslinked?

In order to identify these phosphate groups the chemical crosslinking procedure was carried out. The crosslinking efficiency achieved 30%. The approaches developed can be employed in the studies of pro- and eucaryotic homologs of the E. coli Fpg protein as well as other repair enzymes.

Is EDC soluble in ethanol?

Solubility : Soluble in water (>200 mg/ml), ethanol, DMF, alcohol, and methanol.

What is the difference between DCC and EDC?

The key difference between DCC and EDC is that DCC is a cyclic compound, whereas EDC is an aliphatic compound. Another major difference between DCC and EDC is that DCC is insoluble in water while EDC is water-soluble.

What is the mechanism of reaction of carboxylic acid with EDC xhcl?

Mechanism of reaction of carboxylic acid with EDC.xHCl. Carboxyl group activation with EDC.xHCl proceeds similarly to other carbodiimide couplings. An O-acylisourea intermediate is formed by the reaction of a carboxylic acid and the carbodiimide.

What is EDC coupling in organic chemistry?

EDC coupling – Amides from Amines and Carboxylic Acids In an identical reaction, EDC facilities formation of amides from carboxylic acids: In both cases, the reaction works at room temperature with quite good yields (70-90 %).

What is the mechanism of EDC mediated coupling of carboxylic acids and amines?

Mechanism The scheme above shows the general mechanistic steps for EDC mediated coupling of carboxylic acids and amines under acidic conditions. The tetrahedral intermediate and the aminolysis steps are not shown explicitly. EDC couples primary amines to carboxylic acids by creating an activated ester leaving group.

What happens when EDC reacts with carbonyl carbon?

First, the carbonyl of the acid attacks the carbodiimide of EDC, and there is a subsequent proton transfer. The primary amine then attacks the carbonyl carbon of the acid which forms a tetrahedral intermediate before collapsing and discharging the urea byproduct.