What are MMP-2 and MMP-9?
What are MMP-2 and MMP-9?
Matrix metalloproteinases (MMPs) are a family of zinc-dependent proteases, and MMP-2 and MMP-9 are major members of the MMP family (6,7). MMP-2 and MMP-9 are mainly secreted by tumor cells and stromal cells in the form of zymogens.
What does MMP-2 degrade?
MMP-2, or gelatinase A, is another MMP found to be important in AAA pathogenesis. It too has the capacity to degrade elastin, but unlike MMP-9, it can degrade intact fibrillar collagen.
What are MMP substrates?
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. In stock MMP Substrates are available for immediate shipping. …
What is MMP in pathology?
Matrix metalloproteinases (MMPs) are members of an enzyme family that require a zinc ion in their active site for catalytic activity. MMPs are critical for maintaining tissue allostasis.
What produces mmp9?
Cell Expression of MMP-9 MMP-9 is secreted by a wide number of cell types, including neutrophils, macrophages, and fibroblasts. Neutrophils contain multiple proteases, such as serine proteases (elastase, cathepsin G, and proteinase 3), MMPs (MMP-8 and -9), and urokinase plasminogen activator (uPA).
Is MMP-9 an enzyme?
Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix.
What does MMP-2 stand for?
Assessment of gelatinases (MMP-2 and MMP-9) by gelatin zymography Gelatin zymography is a simple yet powerful method to detect proteolytic enzymes capable of degrading gelatin from various biological sources.
What is the role of MMP in gel zymography?
MMPs within the gel digest the gelatin, resulting in clear bands on a dark blue background after staining with Coomassie. Advantages: gel zymography is an easy and low cost method that provides highly sensitive detection of gelatinase activity 3.
What are the substrate specificities for MMP-9 and MMP2?
However, triple helical substrates that more closely mimic the natural substrate structure have also been developed 9. Substrate specificities for MMP-9 and MMP-2 are similar, making it hard to differentiate activity of the individual enzymes. This issue can be avoided by including a step to isolate either MMP-9 or MMP-2 using antibodies 10.
How can I measure MMP activity in vivo1?
Measuring MMP protein abundance and localization by classic methods such as western blot, ELISA or IHC is not sufficient to accurately describe protein activity in vivo1. Multiple methods have been developed to look at MMP-2 and MMP-9 activity. Find which assay is most appropriate to answer your research questions.
