How does parvovirus B19 replicate?
How does parvovirus B19 replicate?
Human parvovirus B19 (B19V) infection of human erythroid progenitor cells (EPCs) induces a DNA damage response and cell cycle arrest at late S phase, which facilitates viral DNA replication.
What is the structure of canine parvovirus?
The canine parvovirus (CPV) capsid is a T=1 icosahedron that is assembled from 60 copies of combinations of the viral proteins VP1 and VP2. A VP3 form is generated in full (DNA-containing) capsids through the proteolytic cleavage of ∼19 residues from the N terminus of a proportion of the VP2 molecules (15).
Is Parvoviridae enveloped?
Parvoviruses are non-enveloped, icosahedral particles 18 to 26 nm in diameter. Plus and minus DNA strands are packaged into separate virions in approximately equal proportion. There are two capsid proteins.
Is adenovirus an RNA virus?
Adenovirus infection, as is the case with other viruses, could lead to the production of double-stranded RNA (dsRNA) through symmetrical transcription of the viral genome.
Is poliovirus enveloped?
Poliovirus, the causative agent of paralytic poliomyelitis, is a small non-enveloped virus, with a single strand RNA genome encased in an icosahedral protein capsid.
What type of virus is the canine parvovirus?
Background. Canine parvovirus (CPV) is a single-stranded, negative-sense DNA, nonenveloped virus that infects dogs causing loss of appetite, fever, and dehydration leading, in some cases, to death. “Parvo” is highly contagious, but can be prevented by vaccination.
Is canine parvovirus DNA or RNA?
Canine parvovirus (CPV) belongs to the genus Protoparvovirus and the family Parvoviridae and causes a highly contagious and fatal disease in dogs [1]. CPV is a non-enveloped DNA virus with an approximately 5000-nucleotide, single-stranded DNA genome containing two open reading frames (ORFs).
What is the smallest DNA virus?
AAV is the smallest DNA virus with an average size of 20 nm. AAV was discovered in 1965 as a defective contaminating virus in an adenovirus stock (Atchison et al., 1965).
How many genes are in adenovirus?
38 genes
The 38 genes in the Human adenovirus E genome are organized in 17 transcription units, each containing 1-8 coding sequences. Alternative splicing during processing of the pre-mRNAs produced by each transcription unit enable multiple different mRNAs to be produced from one transcription unit.
What is the shape of parvovirus?
Parvoviruses are very small (between 18 and 25 nm in diameter), non-enveloped, single-stranded DNA viruses with an icosahedral capsid. DNA replication of these viruses occurs in the nucleus, and some general replication strategies are employed; for example, the virus needs to make mRNAs that can be translated into proteins…
What is the replication process of parvovirus?
Replication process. Before the start of any replication, parvoviruses must first enter the cell. An attachment to host receptors initiates clathrin-mediated endocytosis of the virion into the host cell.
Can we detect parvovirus B19 in productively infected cells?
Single-stranded DNA, monomeric double-stranded DNA, and dimeric double-stranded DNA forms can all be detected in productively infected cells. The role of the NS1 protein in parvovirus B19 replication was also investigated, but the exact benefits of host cell cycle arrest for this virus remain elusive.
What is the history of parvovirus infection?
The first pathogenic human parvovirus to be discovered was parvovirus B19 in 1974, which became associated with various diseases throughout the 1980s. Parvoviruses were first classified as the genus Parvovirus in 1971 but were elevated to family status in 1975.
