What is chromosomal microarray?

What is chromosomal microarray?

Chromosomal microarray analysis (CMA) is a technology used for the detection of clinically-significant microdeietions or duplications, with a high sensitivity for submicroscopic aberrations.

What is microarray and its types?

Types of microarrays include: DNA microarrays, such as cDNA microarrays, oligonucleotide microarrays, BAC microarrays and SNP microarrays. MMChips, for surveillance of microRNA populations. Protein microarrays. Peptide microarrays, for detailed analyses or optimization of protein–protein interactions.

What is a microarray test used for?

What does chromosomal microarray detect? Chromosomal microarray (CMA) testing looks for extra (duplicated) or missing (deleted) chromosomal segments, sometimes called copy number variants (CNVs).

How is chromosomal microarray testing done?

How the Test is Done. A blood sample is preferred for microarray analysis. Sometimes spit (saliva) or a tissue sample (like skin) may be used. This test compares the patient’s sample to a normal control sample to find very small missing or extra chromosome pieces that cannot be seen under a microscope.

Why do we use microarray?

A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. The DNA molecules attached to each slide act as probes to detect gene expression, which is also known as the transcriptome or the set of messenger RNA (mRNA) transcripts expressed by a group of genes.

What are the steps of microarray?

Steps involved in cDNA based microarray:

• Sample collection.
• Isolation of mRNA.
• Creation of labeled cDNA.
• Hybridization.
• Collection and analysis.

What are the applications of microarray?

The current scope of microarray applications includes sequencing by hybridization, resequencing, mutation detection, assessment of gene copy number, comparative genome hybridization, drug discovery, expression analysis, and immunoassay (protein microarrays).

How is a microarray made?

A typical microarray experiment involves the hybridization of an mRNA molecule to the DNA template from which it is originated. Many DNA samples are used to construct an array. The amount of mRNA bound to each site on the array indicates the expression level of the various genes. This number may run in thousands.

How is microarray made?

How long is chromosomal microarray?

Microarray testing will find common chromosome conditions, like Down syndrome, and can also find chromosome conditions that would not be seen with a karyotype. Microarray results take about two to three weeks.