What is ZORBAX column?
What is ZORBAX column?
The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution.
What is the HPLC column?
This type of HPLC column separates polar molecules based on their charge. The mobile phase is an aqueous buffer. Ion exchange HPLC columns can be used to separate many types of analytes and are commonly used for separations of carbohydrates, amino acids, and proteins. Reversed-phase HPLC columns have nonpolar packing.
When would you use a phenyl hexyl column?
Phenyl-Hexyl Phase Information Luna Phenyl-Hexyl columns offer increased retention for polar, aromatic compounds and can be used for applications that require reversals in elution order. Offers great stability as well as alternative selectivity.
What is difference between C8 and C18?
C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.
What is the best HPLC column for reversed phase?
The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution.
What is the pH range of Agilent zorbax columns?
• Extended pH range, pH 1 to 9. A better way to faster separations! All Agilent Zorbax columns are uniquely optimized for specific applications. Our newest Zorbax columns, Rapid Resolution HT, are packed with totally porous 1.8µm particles.
What is the size of the nanoparticles available for HPLC?
Traditional, fully porous particles available in 1.8, 3.5, and 5 µm sizes. With selected products also available in 7 μm particles for preparative HPLC (see Prep LC columns section)
How does the zorbax extend-c18 column work?
The Agilent ZORBAX Extend-C18 column uses a novel bidentate C18-C18 bonding technology to make it possible to develop high-resolution separations at high pH with a silica-based column. At high pH, non-charged basic compounds will not interact with the underlying silica.