How do I read my EnteroPluri test results?
How do I read my EnteroPluri test results?
Lay EnteroPluri-Test with its flat surface pointing upward and, by punching the plastic film, add 3 or 4 drops of Kovac’s Indole Reagent in the sector H2S/Indole. The reaction is positive if a pink-red colour develops in the added reagent within 10-15 seconds.
Is Citrobacter urease positive or negative?
Biochemical Test and Identification of Citrobacter freundii
| Basic Characteristics | Properties (Citrobacter freundii) |
|---|---|
| Pigment | Negative (-ve) |
| Shape | Rods |
| Spore | Negative (-ve) |
| Urease | Variable |
Is Citrobacter lactose fermenter?
Citrobacter species are differentiated by their ability to convert tryptophan to indole (C. koseri is the only citrobacter to be commonly indole-positive), ferment lactose (C. koseri is a lactose fermentor), and use malonate. Citrobacter shows the ability to accumulate uranium by building phosphate complexes.
What is the purpose of using Enterotube?
The Enterotube system provides a simple, reliable, and rapid method for the presumptive identification of Enterobacteriaceae. The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony.
What tests are in an Enterotube?
The 5 carbohydrates tested in 1 enterotube are: glucose, adonitol, lactose, arabinose, and sorbitol. The formation of acids can be readily detected by including a pH indicator in the microbial growth medium.
Is Citrobacter Koseri urease positive?
koseri. Our strain is also urease positive like other Citrobacter sp.
Is E coli positive for oxidase test?
On the left is oxidase-positive Pseudomonas aeruginosa and on the right is oxidase-negative Escherichia coli.
Is E coli a lactose fermenter?
E. coli are facultative anaerobic, Gram-negative bacilli that will ferment lactose to produce hydrogen sulfide. Up to 10% of isolates have historically been reported to be slow or non-lactose fermenting, though clinical differences are unknown.
Can e Aerogenes ferment lactose?
The pink color of the bacterial growth indicates E. aerogenes is able to ferment lactose. Quadrant 2: Growth on the plate indicates the organism, Escherichia coli, is not inhibited by bile salts and crystal violet and is a gram-negative bacterium. coli is able to ferment lactose.
How long does it take for Enterobacteriaceae to be identified?
Some organisms may require longer than 24 hours incubation for proper identification. Titsworth, E., Grunberg, E., Beskid, G., Cleeland, R., & Delorenzo, W. F. (1969). Efficiency of a multitest system (Enterotube) for rapid identification of Enterobacteriaceae.
What are the characteristics of E coli?
Biochemical Test and Identification of E. coli Characteristics E. coli Gram Staining Negative Shape (Cocci/Diplococci/Rods) Rods Motility (Motile / Non-Motile) Motile Capsule (Capsulated/Non-Capsulated) Variable
Can a false positive E coli test be done with indole test?
Tmax of K. oxytoca was clearly lower than Tmax of K. pneumoniae but a rather high fraction of its isolates produced indole at 44·5°C. Conclusions: False-positive E. coli confirmation is possible if gas production is not tested for and the confirmation is based on indole test only.
How do I interpret the results of BBL Enterotube II?
The Results Pad and Color Reaction Chart permit a rapid check of the positive reactions obtained with BBL Enterotube II. The checked positive numbers are totaled, and the composite number is then located in the Interpretation Guide to identify the organisms.
