What is the composition of carbol fuchsin dye?
What is the composition of carbol fuchsin dye?
Carbol fuchsin, carbol-fuchsin, or carbolfuchsin, is a mixture of phenol and basic fuchsin, used in bacterial staining procedures. It is commonly used in the staining of mycobacteria as it has an affinity for the mycolic acids found in their cell membranes.
How do you make carbol fuchsin stain?
Preparation of reagents. Carbol fuchsin (1%) was prepared from 10 g of basic fuchsin (Hi-Media) dissolved in 100 ml of methanol (Qualigens) and 50 ml of melted phenol (Qualigens) in a flask maintained at 60°C in a water bath. This solution was made up to 1,000 ml with distilled water.
What are the reagents used in acid-fast staining?
The acid-fast bacilli will stain bright red, and the background will stain blue. Reagents used in the procedure include Ziehl–Neelsen carbol-fuchsin solution, 1% acid alcohol, and methylene blue solution [15].
How do you make dilute carbol fuchsin?
Preparation of dilute carbol-fuchsin
- Dissolve 0.3 g basic fuchsin in 10.0 ml 95% ethyl alcohol.
- Add 5.0 ml melted phenol crystals to 95.0 ml distilled water.
- Add the 5% phenol solution to the fuchsin solution and let stand overnight.
- Filter through coarse filter paper.
Is carbol fuchsin used in Gram staining?
Bacteria that retain the initial crystal violet stain (purple) are said to be “gram-positive,” whereas those that are decolorized and stain red with carbol fuchsin (or safranin) are said to be “gram-negative.” This staining response is based on the chemical and structural makeup of the cell walls of both varieties of …
What are the 3 main steps in the acid fast stain?
Acid-Fast Staining Instructions
- Air dry and heat fix a thin film of microorganisms.
- Flood the slide with Carbolfuchsin.
- Flood slide with Acid Alcohol for 30 seconds.
- Counterstain by flooding the slide with Methylene Blue for 30 seconds.
- Dry the slide by putting it between the pages of a book of Bibulous paper.
What 2 genera contain species that are acid-fast organisms?
Mycobacterium and many Nocardia species are called acid-fast because during an acid-fast staining procedure they retain the primary dye carbol fuchsin despite decolorization with the powerful solvent acid-alcohol. Nearly all other genera of bacteria are nonacid-fast.
What is reagent composition of Gram stain?
Reagents needed for Gram staining include: Crystal violet (primary stain) [1] Gram’s iodine solution (the mordant) [1] Acetone/ethanol (50:50 v:v) (the decolorizer) [1] 0.1% basic fuchsin solution (the counterstain) [1]
How do you make Ziehl Neelsen stain?
Ziehl Neelsen Acid-fast stain
- Step 2: Smear Preparation (Review)
- Cover the smear with carbolfuchsin dye.
- Dry heat for 2 minutes.
- Cool and rinse with water.
- Wash the top and bottom of slide with water and clean the slide bottom well.
- Counterstain with Methylene Blue for 30 seconds to 1 minute.
Is carbol Fuchsin used in Gram staining?
How do you prepare carbol fuchsin solution for chromatography?
1. Deparaffinize and hydrate to distilled water. 2. Stain sections with freshly filtered carbol fuchsin solution for 30 min at 37 °C and wash well in running tap water. 3. Decolorize the sections in 1% acid alcohol solution or in 1% aqueous sulfuric acid solution until sections are pale pink.
What are the ingredients in carbol fuchsin?
Carbol Fuchsin (Zn,Strong)S005 Ingredients Basic fuchsin 0.300 gm Ethyl alcohol, 95% 10.000 ml Phenol 5.000 gm Distilled water 95.000 ml **Formula adjusted, standardized to suit performance parameters
Why does carbol fuchsin stain with ferric ferricyanide?
It gives acid-fast coloration with carbol fuchsin. It stains with ferric ferricyanide (Schmorl method I), methyl green, and the periodic acid-Schiff (PAS) reaction. The reason for the PAS-positive reaction in only the intermediate stages of oxidation of lipids is unclear.
How do you clean a smear with carbol fuchsin?
Carbol fuchsin (Ziehl-Neelsen). Methylene blue. Prepare a thin film and fix by heat. Flood the slide with hot strong carbol fuchsin for 1 min. Wash rapidly with tap water and then with ethanol. Treat the smear with solution 2 (Muir’s mordant) for 30 seconds and wash with water.
