What is a normal cell line?
What is a normal cell line?
An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods in vitro.
What is BT-474 cell line?
The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast. Age 60 years Ethnicity White Gender Female Karyotype. The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range.
What is ovcar3?
NIH:OVCAR-3 is an appropriate model system in which to study drug resistance in ovarian cancer, and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.
What are MCF-7 cells used for?
MCF-7 cells are useful for in vitro breast studies because they retained several ideal characteristics particular to mammary epithelium, such as the processing of estrogen, in the form of estradiol, via estrogen receptors (ER) in the cell cytoplasm [28]. It is the first hormone responding breast cancer cell line.
What cell line means?
(Science: cell culture) a cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. No cell lines have been produced from avian tissues and the establishment of cell lines from human tissue is difficult.
What is the difference between a cell and a cell line?
Primary cell culture is the culture of cells directly isolated from parental tissue of interest; whereas cell line is the culture of cells originated from a primary cell culture, which is generally used to expand cell population and prolong life span. However, cell lines have prolonged lifespan.
How do Luminal a T47D and HER2-positive MDA-MB-453 cells differ?
Two-dimensional culture of (a) luminal A T47D and (b) HER2-positive MDA-MB-453 cell lines grown on tissue culture plastic. T47D cells demonstrate a tightly cohesive cobblestone appearance, whereas MDA-MB-453 cells have an elongated and spindly appearance.
What is T47D cell line used for?
T47D cell line human has been used: to study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
What is the best cell line to study HER2 effects?
For example, BT-474 is great to study HER2 effects, while T-47Ds are considered HER2 negative. In order to choose the best cell line for your experiment you should consider the defining facts of your question and choose the cell line by it characteristics and its ability to fit to the system you’re are trying to adress.
Does trastuzumab work in HER2-positive breast cancer cells?
The response to trastuzumab, an antibody that selectively binds HER2, was evaluated in a panel of nine breast cancer cell lines with known HER2 amplification, but only three out of nine cell lines showed an unequivocal response [ 22 ].
