What is Alamar blue used for?
What is Alamar blue used for?
alamarBlue Cell Viability Reagent is an indigo-colored, non-toxic reagent that detects metabolically active cells and is used for the quantitative analysis of cell viability and proliferation.
What is the Alamar blue assay?
alamarBlue is a cell proliferation assay that provides a rapid, sensitive, and economical way to quantitatively measure cell proliferation and cytotoxicity in various human and animal cell lines. It can also be used to study mycobacteria, bacteria, and fungi.
How do you make Alamar blue solution?
Reagent Preparation
- Dissolve alamar blue in PBS (pH 7.4) to 0.15 mg/ml.
- Filter-sterilize the resazurin solution through a 0.2 μm filter into a sterile, light protected container.
- Store the resazurin solution protected from light at 4°C for frequent use or at -20°C for long term storage.
What is Presto blue?
PrestoBlue® is a ready to use cell permeable resazurin-based solution that functions as a cell viability indicator by using the reducing power of living cells to quantitatively measure the proliferation of cells. This color change can be detected using fluorescence or absorbance measurements.
What is cellular viability?
Cell viability is a measure of the proportion of live, healthy cells within a population. Typically, cell viability assays provide a readout of cell health through measurement of metabolic activity, ATP content, or cell proliferation.
What is alamarblue used for?
alamarBlue® is a cell proliferation assay that provides a rapid, sensitive and economical way to quantitatively measure cell proliferation and cytotoxicity in various human and animal cell lines. It can also be used to study mycobacteria, bacteria and fungi.
What does the Alamar Blue cell viability assay measure?
Alamar Blue Cell Viability Assay. AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change color in response to the chemical reduction of growth medium due to cell growth.
What is the color of alamarblue in oxidized form?
The alamarBlue (resazurin) dye in its oxidized form is blue in color and non-fluorescent. In alamarBlue assay the growing cells cause a chemical reduction of the alamarBlue dye from non-fluorescent blue to red fluorescent. The continued growth of viable cells maintain a reducing environment (fluorescent,…
What is the difference between high sensitivity and alamarblue?
High Sensitivity: detects as few as 50 cells. Non-toxic: alamarBlue does not kill cells and is safe to the user and the environment. Simple: water soluble, just add and measure the formula in cell cultures. Flexible: colorimetric and fluorometric detection, for both suspended and attached cell lines.
