What is RP HPLC method?

What is RP HPLC method?

RP-HPLC is a commonly used method for the analysis and purification of peptides, proteins, and glycoproteins. Monosaccharide composition and content can be determined by using the RP-HPLC separation of p-aminobenzoic ethyl ester derivatives of neutral and amino sugars released from glycoproteins.

What is difference between UV and HPLC?

There was no statistically significant difference between the mean values, although UV method showed slightly higher % R.S.D. value compared with HPLC method. Therefore, both analytical methods, were found to be accurate, precise and could be used for routine quality control analysis of repaglinide.

What is impurity HPLC?

High performance liquid chromatography (HPLC) is routinely used for determination of both assay and impurities in both bulk active and formulated drug products. Impurity profile analyses are required to demonstrate the ability to detect a wide range of impurities which may occur in pharmaceuticals.

Can HPLC be used for quantification?

Basically, no. HPLC analysis does not allow for that. Once this is done, you can then analyze your sample using the exact same method, instrument, column on the same day to obtain the amount.

What is the difference between HPLC and RP-HPLC?

The key difference between reverse phase and normal phase HPLC is that the reverse phase HPLC uses a nonpolar stationary phase and a polar mobile phase whereas the normal phase HPLC uses a polar stationary phase and a less polar mobile phase.

Why RP-HPLC is preferred?

RP-HPLC allows purification of most classes of compounds, including compounds present in various herbal products, and is often the most preferable choice when analyzing and attempting to separate and identify compounds from a complex mixture [12].

Why HPLC is more sensitive than UV?

HPLC-UV is GENERALLY much better than UV alone. Samples may be contaminated with other chemicals that absorb at the same wavelength to give a falsely high concentration of desired analyte in a UV protocol. As mentioned above, HPLC also tends to be more sensitive. Both require preparation of a standard curve.

What is pharmaceutical impurity?

An impurity in pharmaceuticals is classified as any component that is not the entity defined as the drug substance. In addition, for a drug product, any component that is not a formulation ingredient is considered an impurity.

What is HPLC PDF?

High performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of pharmaceutical and biological samples. It is the most versatile, safest, dependable and fastest chromatographic technique for the quality control of drug components.

What compounds can be detected by HPLC?

Using the HPLC technique, 11 analytes were identified, and their specificity was confirmed: procyanidin B1, (+)-catechin, chlorogenic acid, procyanidin B2, (−)-epicatechin, rutin, hyperoside, isoquercitrin, avicularin, quercitrin, and phloridzin.

What is the RP-HPLC-UV method?

The RP-HPLC-UV method was developed and validated for the simultaneous determination of three bronchodilators. The method is simple, sensitive and robust. The method was successfully applied for their determination in pure and dosage forms.

What are the advantages of UV over HPLC for pirfenidone?

Moreover, the UV method offers a cost effective and time saving alternative to HPLC method of analysis for pirfenidone from formulations. The HPLC method enables faster quantification of pirfenidone within run time of four minutes without interference of excipients.

What is the linearity of pirfenidone in UV spectrophotometry?

The UV spectrophotometric determinations were performed at 317 nm using methanol as a solvent. The proposed methods were validated according to International Conference on Harmonization ICH Q2 (R1) guidelines. The linearity range for pirfenidone was 0.2-5.0 and 3-25 µg/ml for HPLC and UV method, respectively.