What is the size of bovine serum albumin?
What is the size of bovine serum albumin?
66.5 kDa
According to the literature, BSA has a nominal size of 7.1 nm and a molecular mass of 66.5 kDa. Bovine serum albumin shows a natural tendency to dimerize under stress conditions, with BSA dimers displaying a molecular mass of 132 kDa.
How Big is an albumin molecule?
The glomerular pore size is equal to 4.0–4.5 nm,[5] and the molecular size of albumin is equal to 6.4 nm × 5.5 nm × 5 nm.
How do you get 10% BSA on PBS?
For a 10% (100 mg/mL) stock solution of BSA, dissolve 1 g powdered Fraction V or molecular biology grade BSA in 10 mL of distilled H2O; to avoid clumping, dissolve by layering the powder on the surface of the liquid. Gently rock the capped tube until the BSA has dissolved completely. Do not stir.
What is the structure of BSA?
Bovine serum albumin structure and biological functions The BSA molecule consists of 583 amino acids, bound in a single chain cross-linked with 17 cystine residues (eight disulfide bonds and one free thiol group), and has a molecular mass of 66400 Da [1].
What is the size of BSA?
BSA is a 585 amino acids, 66 kDa globular protein comprised of at least 67% α-helical secondary structure in physiological conditions [15]. It has three main domains which can change conformations under different conditions of pH [16,17].
What is fetal bovine serum used for?
Fetal bovine serum (FBS) is a byproduct of harvesting cattle for the meatpacking industry—it’s used extensively by both academic and industrial researchers as a supplement to basal growth medium in cell culture applications. FBS is the liquid portion that remains after blood is drawn from bovine fetus coagulates.
How is serum albumin measured?
Albumin is generally measured by a dye-binding technique that utilizes the ability of albumin to form a stable complex with bromocresol green dye. The BCG-albumin complex absorbs light at a different wavelength from the unbound dye. This method may overestimate albumin by binding to other proteins.
How do you calculate BSA concentration?
The concentration of BSA in solution can be determined by substituting the molecular weight, extinction coefficient and λmax into a derived form of the Beer-Lambert Law. A substance’s λmax is the wavelength at which it experiences the strongest absorbance. For BSA, this wavelength is 280 nm.
What is the molecular weight (MW) of bovine serum albumin (BSA)?
66.4 kDa. Based on the amino acid sequence, the molecular weight of bovine serum albumin is 66430.3 daltons, which is equivalent to 66430.3 g/mol.
Where does bovine serum come from?
Bovine serum albumin. Bovine serum albumin (also known as BSA or “Fraction V”) is a serum albumin protein derived from cows.
What is normal albumin level in the blood?
Results are given in grams per deciliter (g/dL). A normal albumin range is 3.4 to 5.4 g/dL. If you have a lower albumin level, you may have malnutrition. It can also mean that you have liver disease or an inflammatory disease. Higher albumin levels may be caused by acute infections, burns, and stress from surgery or a heart attack.
What is in bovine colostrum?
Bovine colostrum is a milky fluid that comes from the breasts of cows the first few days after giving birth, before true milk appears. It contains proteins, carbohydrates, fats, vitamins, minerals, and specific kinds of proteins called antibodies that fight disease-causing agents such as bacteria and viruses.
