What is phosphorylated EGFR?

What is phosphorylated EGFR?

Introduction. Epidermal growth factor receptor (EGFR) is involved in regulating cell growth in breast carcinomas. Its activated form, phosphorylated EGFR (pEGFR), is correlated with poor prognosis in lung cancer, but it has not yet been fully investigated in breast cancer.

What is EGFR antibody?

The epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor involved in the proliferation and survival of cancer cells. EGFR is the first molecular target against which monoclonal antibodies (mAb) have been developed for cancer therapy.

What is the molecular weight of EGFR?

130 kDa
Deglycosylation decreases the molecular weight of the EGFR from 170 kDa to 130 kDa (A).

What type of drugs are EGFR inhibitors?

EGFR inhibitors used in NSCLC with EGFR gene mutations

• Erlotinib (Tarceva)
• Afatinib (Gilotrif)
• Gefitinib (Iressa)
• Osimertinib (Tagrisso)
• Dacomitinib (Vizimpro)

Is cetuximab an antibody?

Cetuximab is an IgG(1) monoclonal antibody that blocks EGFR activation. It has efficacy alone, and in combination with irinotecan, in the treatment of metastatic colorectal cancer that has progressed on irinotecan-containing therapy.

How is EGFR activated?

According to the “ligand-induced dimerization model”, EGFR is activated by the ligand-induced dimerization of the receptor monomer, which brings intracellular kinase domains into close proximity for trans-autophosphorylation to initiate downstream signaling cascades.

What are EGFR mutations?

An EGFR mutation refers to a change (damage) to the portion of the DNA in a cell which carries the “recipe” for making EGFR (epidermal growth factor receptor) proteins.

How are antibodies negatively preadsorbed for EGFR inhibition?

The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated epidermal growth factor receptor (EGFR). The final product is generated by affinity chromatography using an EGFR-derived peptide that is phosphorylated at tyrosine 1068.

What is the significance of EGFR phosphorylation at tyr1045?

Background. Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues…

Does tyrosine 1068 cross react with PDGF?

Phospho-EGF Receptor (Tyr1068) Antibody detects endogenous levels of EGF receptor only when phosphorylated at tyrosine 1068. The antibody may cross-react with other activated EGF receptor family members (e.g. ErbB2), and cross-reacts slightly with activated PDGF receptor .

What is the sensitivity of EGF antibody?

Specificity / Sensitivity. Phospho-EGF Receptor (Tyr1068) Antibody detects endogenous levels of EGF receptor only when phosphorylated at tyrosine 1068. The antibody may cross-react with other activated EGF receptor family members (e.g. ErbB2), and cross-reacts slightly with activated PDGF receptor .