What does serine hydrolase do?
What does serine hydrolase do?
Serine hydrolases are involved in a variety of physiological functions, including digestion, immune response, blood coagulation, and reproduction. ABPP has been used recently to investigate host–virus interactions and to understand the molecular pathogenesis of virus infections.
Where are Lyases found?
Lyase-catalyzed reactions break the bond between a carbon atom and another atom such as oxygen, sulfur, or another carbon atom. They are found in cellular processes, such as the citric acid cycle, and in organic synthesis, such as in the production of cyanohydrins.
What role does the serine serve in the oxyanion hole?
The catalytic role of the oxyanion hole in serine protease is generally established to be in stabilizing high-energy intermediates and the transition state through hydrogen bonding.
What is serine hydrolase used for?
Serine hydrolase. Serine hydrolases are one of the largest known enzyme classes comprising approximately ~200 enzymes or 1% of the genes in the human proteome. A defining characteristic of these enzymes is the presence of a nucleophilic serine in their active site, which is used for the hydrolysis of substrates.
What is the nucleophilic serine of hydrolase?
Unlike other non-catalytic serines, the nucleophilic serine of these hydrolases is typically activated by a proton relay involving a catalytic triad consisting of the serine, an acidic residue (e.g. aspartate or glutamate) and a basic residue (usually histidine), although variations on this mechanism exist.
What are the features of the ActivX Tamra-FP serine hydrolase probe?
Features of the ActivX TAMRA-FP Serine Hydrolase Probe: These ActivX™ FP Probes feature a reactive fluorophosphonate group that specifically and covalently labels the active-site serine of enzymatically active serine hydrolases.
