What is anti myc antibody?
What is anti myc antibody?
The Myc tag, derived from the human c-myc gene, is a peptide epitope tag used in detection or purification of recombinant proteins. Antibodies to the myc tag can be used in the analysis of myc-tagged proteins. …
Is myc an antibody?
The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody….Supporting Data.
| REACTIVITY | H M R |
|---|---|
| MW (kDa) | 57 to 70 |
| SOURCE | Rabbit |
What is the purpose of a Myc tag?
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism.
Where is Myc tag from?
The Myc-tag is derived from the human c-myc oncogene and corresponds to amino acid residues 410-419 of the C-terminus of human c-Myc protein. The human c-Myc was discovered as a cellular homolog of v-myc oncogenes, which were identified through analyses of avian tumors1,2.
What is a V5 tag?
The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
Is c-Myc the same as Myc?
The Myc family consists of three related human genes: c-myc (MYC), l-myc (MYCL), and n-myc (MYCN). c-myc (also sometimes referred to as MYC) was the first gene to be discovered in this family, due to homology with the viral gene v-myc. In cancer, c-myc is often constitutively (persistently) expressed.
How do I clean myc tag protein?
Purifying Myc-tagged proteins using immunoprecipitation Immunoprecipitation (IP) is a technique for separating a protein of interest from a solution containing thousands of different proteins, such as a cell lysate, by precipitating it from the solution using an antibody that binds specifically to that protein.
What is the purpose of a myc tag?
What is a Myc DDK tag?
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
What is the V5 epitope?
Is C-MYC a tumor suppressor gene?
Induced overexpression of the c-Myc gene is responsible for many of the changes that induce malignant changes (see Figure 1). These changes support the production of intermediates for cell growth and division, and are regulated by both oncogenes and tumor suppressor genes in a number of key cancer-producing pathways.
How is Myc regulated?
In fact, MYC is not only regulated by a whole host of transcription factors, such as CNBP, FBP, and TCF that is downstream of the Wnt pathway, but it is also regulated by non-B DNA structures including single-stranded bubbles, G-quadruplexes and Z-DNA (Levens, 2010).
