What are MDA-MB-231 cells?

What are MDA-MB-231 cells?

The MDA-MB-231 cell line is an epithelial, human breast cancer cell line that was established from a pleural effusion of a 51-year-old caucasian female with a metastatic mammary adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical research laboratories.

Is MDA-MB-231 basal like?

Our analysis indicates that although MDA-MB-231 was classified as Basal-like subtype and bears the triple-negative phenotype, its cell type of origin may not be Basal cell, which could partially explain why its genomic profile is substantially different from Basal-like MET500 breast cancer samples.

Are MDA-MB-231 metastatic?

MiRNA profiling of the MDA-MB-231 cell line was compared to its derivatives that are highly metastatic to bone and lung.

How do you thaw MDA MB 231 cells?

Thaw the frozen cryovial of cells within 1–2 minutes by gentle agitation in a 37°C water bath. Decontaminate the cryovial by wiping the surface of the vial with 70% (v/v) ethanol. 2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.

Is MCF7 triple negative?

MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.

How long does it take for MDA-MB-231 cells to adhere?

Just aspirate the media and add more. After this, you should see some cells happily attached. Do not change the media or split cells before they are about 60% confluent (which can take 5-7 days for MDA-MB-231, depending on how many survive thawing), unless there are a lot of dead cells floating.

What media does MDA-MB-231 use?

S100A4 gene expression was significantly greater in MDA-MB-231 cells grown in DMEM and RPMI-1640 in comparison to cells grown in MEM and this was also validated by RT-PCR (Fig. 4B).

What does MDA-MB-231 stand for?

MDA-MB-231 (ECACC catalogue no. 92020424) Cell line history. The MDA-MB-231 cell line is aepithelial,n human breast cancer cell line that was established from a pleural effusion year-old caucasian femaleof a 51 with a metastatic mammary – adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical research laboratories.

What is the phenotype of the MDA-MB-231 cell line?

The MDA-MB-231 breast cancer cell line was obtained from a patient in 1973 at M. D. Anderson Cancer Center. With epithelial-like morphology, the MDA-MB-231 breast cancer cells appear phenotypically as spindle shaped cells. In vitro, the MDA-MB-231 cell line has an invasive phenotype.

What is the MDA-MB-231 Vim RFP reporter cell line?

The MDA-MB-231 VIM RFP reporter cell line provides a convenient and sensitive platform for research on the mechanisms of metastasis in vitro and the development of new anti-EMT drugs for metastatic breast cancer.

What is the history of the mb231 cell line?

Cell line history. The MDA-MB-231 cell line is aepithelial,n human breast cancer cell line that was established from a pleural effusion year-old caucasian femaleof a 51 with a metastatic mammary – adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical research laboratories.