What is an anti-GFP antibody?

What is an anti-GFP antibody?

The target antigen refers to green fluorescent protein (GFP), commonly used as an epitope tag for recombinant proteins for detection via immunofluorescence, flow cytometry or western blot. …

Does GFP antibody recognize YFP?

YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. Aequorea victoria. Recognizes native and denatured forms of jellyfish GFP and its variants such as: EGFP, YFP, EYFP, and CFP.

Can GFP antibody detect CFP?

Q: What GFP variants will this antibody recognize? A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.

What is the function of EGFP?

The EGFP mice expressed high levels of urinary 3-ureidopropionate, which is due to the down-regulated transcriptional level of β-ureidopropionase. The expression of EGFP in vivo also affects other metabolic pathways, including nucleic acid metabolism, energy utilization, and amino acids catabolism.

What does the new full-length GFP polyclonal antibody detect?

The new Full-Length GFP Polyclonal Antibody provides equivalent performance to our previous Full-Length A.v. Polyclonal Antibody. The Full-Length GFP Polyclonal Antibody detects AcGFP1, wild type GFP, and GFPuv. Based on their homology to GFP, we also expect this antibody to detect EGFP, EYFP, and ECFP.

What does racgfp1 stand for?

Lanes 4.0.2 ng rAcGFP1. Lanes 5.0.1 ng rAcGFP1. The Living Colors Full-Length GFP Polyclonal Antibody was raised in a rabbit against recombinant full-length Aequorea coerulescens green fluorescent protein (rAcGFP1).

Which antibodies are used to detect reef coral fluorescent proteins?

Western blot detection of Reef Coral Fluorescent Proteins by the Anti-RCFP Polyclonal Pan Antibody. This antibody detects all of the Reef Coral Fluorescent Proteins (AmCyan, AsRed, DsRed2, DsRed-Express, HcRed, ZsGreen, and ZsYellow). It does not recognize green fluorescent protein variants derived from Aequorea species (including AcGFP1).

What does the Pan antibody recognize?

This pan antibody recognizes denatured forms of AmCyan, ZsGreen, ZsYellow, DsRed2, DsRed-Express, HcRed, AsRed, as well as fusion proteins containing these RCFP variants in bacterial and mammalian cell lysates. Our products are to be used for Research Use Only.

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