What is a hotspot panel?
What is a hotspot panel?
Hotspot cancer panels target regions of known cancer genes that have been well-characterized as mutational hotspots. The small target region size produces a high depth of sequencing coverage per run, even when multiplexing many samples together.
What is Comprehensive cancer Panel?
The Comprehensive Panel is a comprehensive 73-gene panel that identifies inherited cancer risks across all major organ systems, giving you more information to make better treatment and management decisions.
What is AmpliSeq?
AmpliSeq chemistry allows researchers to analyze hundreds of genes simultaneously with ultra-high multiplexed PCR with 12 to more than 24,000 amplicons in a single panel. Achieve complete coverage of large targets using multiple primer pools to create overlapping amplicons.
What is AmpliSeq library?
The Ion AmpliSeq™ Library Kit 2.0 is designed for rapid preparation of amplicon libraries using Ion AmpliSeq™ panels. Scalable Multiplex PCR Reactions. The Ion AmpliSeq 2.0 technology enables scalable multiplex PCR reactions from 12- to 24,000-plex in a single well using just 10 ng of starting DNA.
What is ion AmpliSeq designer?
Ion AmpliSeq Designer is an online primer design tool that lets you create custom panels for Ion Torrent next-generation sequencing.
Is amplicon a primer?
Summary: Amplicon is a program for designing PCR primers on aligned groups of DNA sequences. The most important application for Amplicon is the design of ‘group-specific’ PCR primer sets that amplify a DNA region from a given taxonomic group but do not amplify orthologous regions from other taxonomic groups.
What is ion AmpliSeq chemistry?
At its most basic level, an Ion AmpliSeq™ panel consists of a pool of oligonucleotide primer pairs, each pair designed to amplify a specified genomic region. In contrast, a mutation hotspot design typically results in non-overlapping amplicons whose primers can be accommodated in a single multiplexed reaction.
What does the ion chef do?
The Ion Chef System provides reproducible chip loading by automating multiple manual chip-loading steps. The sophisticated visual system inside the Ion Chef System is designed to help reduce user-introduced error and variability by providing barcode reading and positive identification of samples, chips, and reagents.
How big should qPCR amplicons be?
70-200 bp
Typical qPCR amplicons are 70-200 bp in length, and we recommend designing your target in that range. Longer amplicons can be used, but may require optimization (e.g., increased extension times).
