How do you dissolve a Pluronic f127?
How do you dissolve a Pluronic f127?
Dissolve 2 g of Pluronic F-127 in 10 mL of anhydrous dimethylsulfoxide (DMSO) to give a 20% (w/v) stock solution. This may require heating at ~40° for about 20 minutes. Store both solid and solution at room temperature. Do not refrigerate or freeze the solutions because Pluronic F-127 may come out of solution.
What is Pluronic water?
Pluronic F-127 *10% solution in water* is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media.
Is pluronic water soluble?
Pluronics The pluronic PEO block is hydrophilic and water soluble while the PPO block is hydrophobic and water insoluble. In an aqueous environment, these block copolymers self-assemble into micelles with a hydrophobic PPO center core and a hydrophilic PEO outer shell that interfaces with water.
What is Pluronic f68?
Gibco® Pluronic® F-68 is a non-ionic surfactant used to control shear forces in suspension cultures. Pluronic® F-68 can also be used to reduce foaming in stirred cultures and reduce cell attachment to glass. Pluronic® F-68 is provided at a concentration of 10% and effective at a working concentration of 0.1%.
How do you make Pluronic gel?
Preparation of gel Briefly, F127 (1.8 g) was added to 10 mL PBS (pH 7.4) aqueous solution in flat-bottomed screw-capped glass vials, and gently mixed with magnetic stirrers for 24 hours at 4°C until all of the Pluronic granules were completely dissolved and a clear solution was obtained.
Is Pluronic FDA approved?
Pluronics (PEOx-PPOy-PEOx) are non-ionic triblock copolymers composed of two hydrophilic side chains of poly (ethylene oxide), PEO, and a central hydrophobic chain of poly (propylene oxide), PPO. They are also known as poloxamers and are non-toxic, biodegradable and FDA approved [22].
How do you dissolve Pluronic water?
Dissolve 1 g of Pluronic® F-127 in 10 mL distilled water to make a 10% (w/v) stock solution, or 2 g of Pluronic® F-127 in 10 mL of anhydrous dimethyl sulfoxide (DMSO) to make a 20% (w/v) stock solution. These may require heating from 40 to 50 °C for about 30 minutes. Store solution at room temperature.
Is poloxamer 188 same as Pluronic F68?
Pluronic® F68 surfactant. Pluronic® F68 BioReagent is a non-ionic surfactant. It is suitable for cell culture and reagent culture. It is useful to control shear forces in suspension cultures, reduce foaming in stirred cultures, and reduce cell attachment to glass….Pluronic F 68 (Poloxamer 188) 100 g.
| Weight | 100.0 g |
|---|---|
| Weight | 100 g. |
Is Pluronic a detergent?
Pluronic® F-127 is a non-ionic detergent useful for isolation of membrane proteins. It has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media.
What is Pluronic f127 used for?
Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.
Is Pluronic biodegradable?
It is injectable and has a reversible mechanism of gelation [25]. Moreover, Pluronic F-127 hydrogel has favorable properties such as non-toxicity, biocompatibility, and biodegradability.
