What is DEAE ion exchange chromatography?
What is DEAE ion exchange chromatography?
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). In solution, the resin is coated with positively charged counter-ions (cations).
How does an anion exchange column work?
Anion Exchange Column Design Ion exchange chromatography is used to separate charged molecules. In an anion exchange column, the packing is positively charged and therefore retains negatively charged molecules by coulombic interaction. The bound molecules are eluted with an anion gradient.
Which protein elutes first in anion exchange?
If a buffer containing more than one protein is used with an anion exchange resin, then the most negatively-charged protein will be most attracted to the stationary phase and will therefore elute last and the protein with the highest positive charge will elute first.
What is DEAE cellulose chromatography?
Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.
How do you elute protein from cation exchange?
The Technique
- An impure protein sample is loaded into the ion exchange chromatography column at a particular pH.
- Charged proteins will bind to the oppositely charged functional groups in the resin.
- A salt gradient is used to elute separated proteins.
- Unwanted proteins and impurities are removed by washing the column.
What is dedeae Sepharose fast flow?
DEAE Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups.
What is DEAE-Sepharose column?
Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose. Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
What is hihitrap DEAE FF?
HiTrap DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
What type of agarose is used in fast flow column chromatography?
Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose.
