Does pseudouridine block translation?
Does pseudouridine block translation?
Here we used in vitro biochemical and structural studies together with cell-based assays to demonstrate that pseudouridine impedes translation elongation and increases the occurrence of amino acid substitutions.
Does mRNA have pseudouridine?
It has long been known that pseudouridine (Ψ) is the most abundant modified nucleotide in stable RNAs, including tRNA, rRNA, and snRNA. Recent studies using massive parallel sequencing have uncovered the presence of hundreds of Ψs in mRNAs as well. However, the functional role of Ψ in mRNA remains to be elucidated.
What does pseudouridine base pair with?
The base pairing of (a) uridine with adenosine and pseudouridine with (b) adenosine, (c) guanosine and (d, e) uridine. Ψ is found in the TΨC loop of almost all tRNAs. For most tRNAs, Ψ is also found in the D stem and/or in the anticodon stem and loop (1).
Why is Pseudouridine used in mRNA?
Pseudouridine in rRNA and tRNA has been shown to fine-tune and stabilize the regional structure and help maintain their functions in mRNA decoding, ribosome assembly, processing and translation. Pseudouridine in snRNA has been shown to enhance spliceosomal RNA-pre-mRNA interaction to facilitate splicing regulation.
Which bond is present in Pseudouridine?
Pseudouridine is a C5-glycoside isomer of uridine and the only C–C bond joining a nucleobase to a sugar known in nucleic acids [95,97].
What is the meaning of Pseudouridine?
Pseudouridine (abbreviated by the Greek letter psi- Ψ) is an isomer of the nucleoside uridine in which the uracil is attached via a carbon-carbon instead of a nitrogen-carbon glycosidic bond. Pseudouridine is the most abundant RNA modification in cellular RNA.
Is Pseudouridine naturally occurring?
5.1 Principle. Pseudouridylation is a natural modification occurring on uridine base in order to convert it into a pseudouridine (named 5-ribosyluracil, Ψ) (Fig. 3.10). Different classes of RNAs are subject to pseudouridylation, such as tRNAs, ribosomal RNAs (rRNAs), and small nuclear RNAs (snRNAs).
Why is Pseudouridine important?
